The energy transfer fluorescence method for determination of trace phosphorous with acridine orange-rhodamine 6G

被引：0

作者：

Li, BS ^{[1
]}

Wang, GH ^{[1
]}

Sun, HW ^{[1
]}

Bao, SY ^{[1
]}

Huang, YZ ^{[1
]}

机构：

[1] Hebei Univ, Res Ctr Phys & Chem Anal, Baoding 071002, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 29卷 / 01期

关键词：

phosphorous; acridine orange; rhodamine; 6G; energy transfer; fluorescence;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

It was found that at the condition of lambda (ex)/lambda (em) = 450/556 nm, in the dodecyl benzene sodium sulfonate solution, the effective energy transfer could occur between AO and R6G. The fluorescence intensity of R6G was diminished by molybdophosphate which was formed by the reaction between orthophosphate and molybdate in hydrochloric acid medium. The detection limit of this method was 5 ng/L. The range of determination for phosphorous was 0.05 similar to 0.70 mug/L. The relative standard deviation for the determination of 0.5 mug/L phosphorous was 6% (n = 13). The recoveries for the addition of 0.2 similar to 0.4 mug/L phosphorous were 95% similar to 110%. The method has been applied to the determination of phosphorous in water samples and soil samples with satisfactory reuslts.

引用

页码：42 / 44

页数：3