Effects of sulfamethoxazole on the denitrifying process in anoxic activated sludge and the responses of denitrifying microorganisms

被引：18
作者：
An, Yonglei [1 ,2 ,3 ,4 ]
Qin, Xueming [1 ,2 ,3 ]
机构：
[1] Jilin Univ, Key Lab Groundwater Resources & Environm, Minist Educ, Changchun, Jilin, Peoples R China
[2] Jilin Univ, Jilin Prov Key Lab Water Resources & Environm, Changchun, Jilin, Peoples R China
[3] Jilin Univ, Coll New Energy & Environm, Changchun 130021, Jilin, Peoples R China
[4] Hebei & China Geol Survey, Key Lab Groundwater Remediat, Shijiazhuang 050061, Hebei, Peoples R China
来源：
WATER SCIENCE AND TECHNOLOGY | 2018年 / 78卷 / 05期
关键词：
anoxic activated sludge; antibiotic; denitrifying gene; denitrifying microorganisms; gene expression; PSEUDOMONAS-STUTZERI PCN-1; VETERINARY ANTIBIOTICS; MICROBIAL COMMUNITY; AEROBIC DENITRIFICATION; COASTAL SEDIMENTS; MARINE-SEDIMENTS; N2O RELEASE; ENVIRONMENT; EXPOSURE; WATER;
D O I：
10.2166/wst.2018.394
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The presence of antibiotics in municipal wastewater is bound to affect the anoxic denitrifying process in anoxic activated sludge (AAS). This study investigated the effects of sulfamethoxazole (SMZ) on the denitrifying process in AAS and the responses of denitrifying microorganisms. The results showed that SMZ could decrease the speed of nitrate removal significantly when the concentration of SMZ was lower than 10 mg/L, and the removal of nitrate would be completely inhibited when SMZ concentration was higher than 100 mg/L. Weak alkaline condition would enhance the inhibition effect of SMZ on removal of nitrate in the anoxic bioreactor. The results of high-throughput sequencing and qPCR (quantitative polymerase chain reaction) showed that 100 mg/L of SMZ did not decrease the total abundance of denitrifying microorganisms. However, the relative expression levels of key denitrifying genes NirS and NosZ in AAS treated by 100 mg/L of SMZ versus the raw AAS without SMZ was only 0.030 and 0.036. Therefore, the inhibitory mechanism of SMZ on the denitrifying process in AAS was denoted by an effective inhibition to the expressions of denitrifying genes, rather than a decrease in the total abundance of denitrifying microorganisms.
引用
收藏
页码：1228 / 1236
页数：9
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