The use of epifluorescence microscopy and fluorescent dyes for visualization of Oxyuris equi eggs

被引：7

作者：

Barros, Helio L. ^{[1
]}

Marques, Sandra M. T. ^{[2
]}

Stefani, Valter ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Lab New Organ Mat & Forens Chem LNMO QF, Inst Chem, Ave Bento Goncalves,9-500,CP 15003, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Vet, Dept Patol Clin Vet, Lab Helmintol, Ave Bento Goncalves 9090, BR-91501970 Porto Alegre, RS, Brazil

来源：

VETERINARY PARASITOLOGY | 2016年 / 226卷

关键词：

Benzazole dyes; Biomarkers; Fluorescent probes; Pinworm infection; Oxyuris equi; ENTEROBIUS-VERMICULARIS; INFECTIONS; BRAZIL;

D O I：

10.1016/j.vetpar.2016.06.039

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study presents a new method for visualization of Oxyuris equi eggs collected by means of a clear adhesive tape applied to the perianal region of horses. The obtained results indicate that this protocol permits a quick, easy, clear and selective visualization of Oxyuris equi eggs even in the presence of feces and other material. Another advantage of this method is that it can be used with fluorescent dyes solubilized in water, which will stain biologic material without dissolving or altering the adhesive tape and is also environmentally safe. Other dyes currently used for staining biologic materials use organic solvents, which may be combined with acids or bases in their formulation, preventing their use with the tape method. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：162 / 166

页数：5

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