This paper examines how noise in observed option prices arising from discrete prices and other microstructural frictions affects empirical tests of option pricing models and risk-neutral density estimation. The discrete tick size alone introduces enough noise to make model comparisons difficult, especially for lower-priced stocks. We demonstrate that microstructural noise can lead to incorrect inferences in the univariate diffusion test of Bakshi et al. (Rev Financ Stud 13: 549-584, 2000), the transition density diffusion test of Ait-Sahalia (J Financ 57:2075-2112, 2002), and the speed-of-convergence test of Carr and Wu (J Financ 58:2581-2610, 2003). We also show that microstructural noise induces a bias into the implied risk-neutral moment estimators of Bakshi et al. (Rev Financ Stud 16:101-143, 2003). Even in active, liquid option markets, observation error is likely to reduce significantly the power of tests, and in some cases represents an important source of bias.