Micro-CT versus synchrotron radiation phase contrast imaging of human cochlea

被引：52
作者：
Elfarnawany, M. [1 ]
Alam, S. Riyahi [1 ]
Rohani, S. A. [2 ]
Zhu, N. [3 ]
Agrawal, S. K. [1 ,2 ,4 ,5 ]
Ladak, H. M. [1 ,2 ,4 ,5 ]
机构：
[1] Western Univ, Dept Otolaryngol Head & Neck Surg, London, ON, Canada
[2] Western Univ, Biomed Engn Grad Program, London, ON, Canada
[3] Univ Saskatchewan, Canadian Light Source Inc, Biomed Imaging & Therapy Facil, Saskatoon, SK, Canada
[4] Western Univ, Dept Med Biophys, London, ON, Canada
[5] Western Univ, Dept Elect & Comp Engn, London, ON, Canada
来源：
JOURNAL OF MICROSCOPY | 2017年 / 265卷 / 03期
基金：
加拿大健康研究院; 加拿大自然科学与工程研究理事会; 加拿大创新基金会;
关键词：
Human cochlea; image segmentation; microcomputed tomography; phase contrast imaging; synchrotron radiation; three-dimensional model; SCALA-TYMPANI; MEMBRANE; TOMOGRAPHY; MORPHOLOGY; INSERTION; POSITION; GANGLION; PLATFORM; TRAUMA; ARRAY;
D O I：
10.1111/jmi.12507
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
High-resolution images of the cochlea are used to develop atlases to extract anatomical features from low-resolution clinical computed tomography (CT) images. We compare visualization and contrast of conventional absorption-based micro-CT to synchrotron radiation phase contrast imaging (SR-PCI) images of whole unstained, nondecalcified human cochleae. Three cadaveric cochleae were imaged using SR-PCI and micro-CT. Images were visually compared and contrast-to- noise ratios (CNRs) were computed from n = 27 regions-of- interest (enclosing soft tissue) for quantitative comparisons. Three-dimensional (3D) models of cochlear internal structures were constructed from SR-PCI images using a semiautomatic segmentation method. SR-PCI images provided superior visualization of soft tissue microstructures over conventional micro-CT images. CNR improved from 7.5 +/- 2.5 inmicro-CT images to 18.0 +/- 4.3 inSR-PCI images (p < 0.0001). The semi-automatic segmentations yielded accurate reconstructions of 3D models of the intracochlear anatomy. The improved visualization, contrast and modelling achieved using SR-PCI images are very promising for developing atlas-based segmentation methods for postoperative evaluation of cochlear implant surgery.
引用
收藏
页码：349 / 357
页数：9
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