Investigations and comparative detection of Cryptosporidium species by microscopy, nested PCR and LAMP in water supplies of Ordu, Middle Black Sea, Turkey

被引：27
作者：
Koloren, Z. [1 ]
Sotiriadou, I. [2 ,3 ]
Karanis, P. [3 ]
机构：
[1] Univ Ordu, Dept Biol, Fac Arts & Sci, Ordu, Turkey
[2] Univ Cologne, Ctr Dent Med, Policlin Operat Dent & Periodontol, D-50931 Cologne, Germany
[3] Univ Cologne, Ctr Anat, Inst 2, Sch Med, D-50931 Cologne, Germany
来源：
ANNALS OF TROPICAL MEDICINE AND PARASITOLOGY | 2011年 / 105卷 / 08期
关键词：
MEDIATED ISOTHERMAL AMPLIFICATION; PROTOZOAN PARASITES; TOXOPLASMA-GONDII; GIARDIA; OOCYSTS; SAMPLES; OUTBREAKS; TRANSMISSION; PURIFICATION; SURFACE;
D O I：
10.1179/2047773211Y.0000000011
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
A total of 70 water samples, including tap, river, fountain and well water were collected in the Ordu province, Middle Black Sea, Turkey and investigated for the detection of Cryptosporidium oocysts. The samples were directly screened microscopically for Cryptosporidium oocysts' detection by immunofluorescence test and subsequently DNA was extracted for the molecular detection by loop-mediated isothermal amplification (LAMP) and nested polymerase chain reaction (PCR). Eighteen out of the 70 (25.7%) water samples were found positive for Cryptosporidium spp. by immunofluorescence test and 19 (27.1%) were found positive by LAMP. Nested PCR products were not generated in any of the investigated water samples. A total of 16 randomly selected pellets were spiked with 10 Cryptosporidium oocysts to test the efficiency of the applied method. All the samples were found positive by LAMP for the presence of Cryptosporidium DNA, while the nested PCR assay was positive in only seven (43.75%) out of the 16 examined spiked samples. This is the first report on the occurrence of Cryptosporidium species in environmental and drinking water supplies in the Black Sea area.
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收藏
页码：607 / 615
页数：9
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