Detection of single nucleotide mutations in wheat using single strand conformation polymorphism gels

被引：62
|
作者：
Martins-Lopes, P
Zhang, H
Koebner, R
机构：
[1] John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England
[2] Univ Tras Montes & Alto Douro, ICETA, Dept Genet & Biotecnol, P-5001 Vila Real, Portugal
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2001年 / 19卷 / 02期
关键词：
SNP; SSCP; wheat;
D O I：
10.1007/BF02772158
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We show that single strand conformation polymorphism (SSCP) analysis, using the mutation detection enhancement (MDE(TM)) matrix, is efficient at detecting sequence polymorphisms in PCR amplicons. Four independent wheat genomic fragments were amplified from two contrasting templates and sequenced. The allelic fragments were differentiated at 1-6 single nucleotide positions, but MDE-SSCP was able to unequivocally distinguish each allelic pair. The approach is therefore considered a powerful way of identifying single nucleotide polymorphisms (SNPs) without extensive amplicon sequencing.
引用
收藏
页码：159 / 162
页数：4
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