Umbilical cord mesenchymal stem cells suppress B-cell proliferation and differentiation

被引：63
作者：
Che, Nan [2 ]
Li, Xia [2 ]
Zhou, Shiliang [3 ]
Liu, Rui [2 ]
Shi, Dongyan [3 ]
Lu, Liwei [1 ,4 ]
Sun, Lingyun [2 ]
机构：
[1] Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
[2] Nanjing Med Univ, Drum Tower Clin Med Coll, Dept Immunol & Rheumatol, Nanjing, Jiangsu, Peoples R China
[3] Nanjing Univ, Sch Med, Affiliated Drum Tower Hosp, Dept Immunol & Rheumatol, Nanjing, Jiangsu, Peoples R China
[4] Univ Hong Kong, Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China
来源：
CELLULAR IMMUNOLOGY | 2012年 / 274卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Umbilical cord mesenchymal stem cells; B cells; B-cell proliferation and differentiation; HUMAN BONE-MARROW; GERMINAL-CENTER FORMATION; REPRESSION; TRANSPLANTATION; ACTIVATION; PAX5; TRANSCRIPTION; EXPRESSION; BLIMP-1; MODEL;
D O I：
10.1016/j.cellimm.2012.02.004
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Mesenchymal stem cells (MSCs) may be obtained from umbilical cord as an abundant and noninvasive source. However, the immunomodulatory properties of umbilical cord-MSCs (UC-MSCs) were poorly studied. In this study, we aimed to investigate the effects of UC-MSCs on B-cell proliferation and differentiation. UC-MSCs were found to suppress the proliferation of B cells isolated from murine spleen. Moreover, UC-MSCs markedly suppressed B-cell differentiation as shown by the decreased number of CD138 + cells and reduced levels of IgM and IgG production in coculture. As revealed by transwell experiments, soluble factors produced by UC-MSCs might be involved in mediating B-cell suppression. The Blimp-1 mRNA expression was suppressed whereas the PAX-5 mRNA expression was induced in coculture. Finally, UC-MSCs modified the phosphorylation pattern of Akt and p38 pathways, which were involved in B-cell proliferation and differentiation. These results may further support the potential therapeutic use of UC-MSCs in treating autoimmune disorders. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：46 / 53
页数：8
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