Intravital Imaging Techniques for Biomedical and Clinical Research

被引:42
作者
Coste, Anouchka [1 ,2 ,3 ]
Oktay, Maja H. [2 ,3 ,4 ,5 ]
Condeelis, John S. [1 ,2 ,3 ,4 ]
Entenberg, David [2 ,3 ,4 ]
机构
[1] Montefiore Med Ctr, Einstein Coll Med, Dept Surg, Bronx, NY 10467 USA
[2] Montefiore Med Ctr, Einstein Coll Med, Dept Anat & Struct Biol, 111 E 210th St, Bronx, NY 10467 USA
[3] Montefiore Med Ctr, Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10467 USA
[4] Montefiore Med Ctr, Einstein Coll Med, Integrated Imaging Program, Bronx, NY 10467 USA
[5] Montefiore Med Ctr, Einstein Coll Med, Dept Pathol, Bronx, NY 10467 USA
关键词
multiphoton; intravital; imaging windows; clinical applications; IN-VIVO;
D O I
10.1002/cyto.a.23963
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Intravital imaging, the direct visualization of cells and tissues within a living animal, is a technique that has been employed for the better part of a century. The advent of confocal and multiphoton microscopy has dramatically improved the power of intravital imaging, making it possible to obtain optical sections of tissues non-destructively. This review discusses the various techniques used for intravital imaging, describes how intravital imaging provides information about cellular and tissue dynamics not possible to be garnered by other techniques, and details several ways in which intravital imaging is making a direct impact on the clinical care of patients. (c) 2019 International Society for Advancement of Cytometry
引用
收藏
页码:448 / 457
页数:10
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