Rapid screening of Mycobacterium tuberculosis complex (MTBC) in clinical samples by a modular portable biosensor

被引：29

作者：

Prabowo, Briliant Adhi ^{[1
,2
]}

Chang, Ying-Feng ^{[3
]}

Lai, Hsin-Chih ^{[4
,5
,6
,7
]}

Alom, Azharul ^{[1
]}

Pal, Parthasarathi ^{[1
]}

Lee, Yu-Ying ^{[8
]}

Chiu, Nan-Fu ^{[9
]}

Hatanaka, Koji ^{[10
]}

Su, Li-Chen ^{[11
]}

Liu, Kou-Chen ^{[1
,5
,12
,13
]}

机构：

[1] Chang Gung Univ, Dept Elect Engn, Taoyuan 33302, Taiwan

[2] Indonesian Inst Sci, Res Ctr Informat, Bandung 40135, Indonesia

[3] Natl Taiwan Univ, Dept Biochem Sci & Technol, Taipei 10617, Taiwan

[4] Chang Gung Univ, Coll Med, Dept Med Biotechnol & Lab Sci, Taoyuan 33302, Taiwan

[5] Chang Gung Mem Hosp, Dept Lab Med, Taoyuan 33305, Taiwan

[6] Chang Gung Univ Sci & Technol, Res Ctr Ind Human Ecol, Taoyuan 33302, Taiwan

[7] Chang Gung Univ Sci & Technol, Grad Inst Hlth Ind Technol, Taoyuan 33302, Taiwan

[8] Chang Gung Univ, Grad Inst Electroopt Engn, Taoyuan 33302, Taiwan

[9] Natl Taiwan Normal Univ, Inst Electroopt Sci & Technol, Taipei 10610, Taiwan

[10] Acad Sinica, Res Ctr Appl Sci, Taipei 11529, Taiwan

[11] Chinese Culture Univ, Dept Optoelect Phys, Taipei 11114, Taiwan

[12] Chang Gung Univ, Ctr Biomed Engn, Taoyuan 33302, Taiwan

[13] Ming Chi Univ Technol, Dept Mat Engn, New Taipei 24301, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 254卷

关键词：

Surface plasmon resonance (SPR); Organic light emittingdiode (OLED); Portable biosensor; Tuberculosis; DNA; Polymerase chain reaction (PCR); PLASMON RESONANCE IMMUNOSENSOR; REAL-TIME PCR; LABEL-FREE; IDENTIFICATION; DNA; ASSAY; MIESCHER; FRIEDRICH; SENSITIVITY; PERFORMANCE; DIAGNOSIS;

D O I：

10.1016/j.snb.2017.07.102

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Mycobacterium tuberculosis complex (MTBC) is the well-known causative agents of tuberculosis (TB). Transmission of bacteria via airborne routes makes infection control difficult. Hence, rapid and accurate identification of TB from clinical sputum samples is essential for minimizing the spread of the disease. However, traditional bacterial identification methods for TB are time consuming and labor intensive. The aim of this study is to propose a straightforward and rapid screening platform for MTBC using a portable organic light-emitting diode- (OLED-) based surface plasmon resonance (SPR) biosensor integrated with a nested PCR technique. Experimentally, the limit of detection (LOD) for MTBC PCR products measurement was estimated to be around 63 pg/mL. In addition, the biosensor can successfully differentiate MTBC from other nontuberculous mycobacterium strains. In 600 clinical sputum specimens, the sensitivity and specificity were 96.6% (86/89) and 98.4% (503/511), respectively. These results indicate that the portable OLED-based SPR biosensor may be taken into account as a suitable and convenient tool employed in the remote area and developing country for diagnosing TB diseases. (C) 2017 Published by Elsevier B.V.

引用

页码：742 / 748

页数：7

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