In vitro anti-trypanosomal activity of elatol isolated from red seaweed Laurencia dendroidea

被引：46

作者：

Veiga-Santos, P. ^{[1
]}

Pelizzaro-Rocha, K. J. ^{[2
]}

Santos, A. O. ^{[2
]}

Ueda-Nakamura, T. ^{[1
,3
]}

Dias Filho, B. P. ^{[1
,3
]}

Silva, S. O. ^{[1
,3
]}

Sudatti, D. B. ^{[4
]}

Bianco, E. M. ^{[5
]}

Pereira, R. C. ^{[4
]}

Nakamura, C. V. ^{[1
,3
]}

机构：

[1] Univ Estadual Maringa, Programa Posgrad Ciencias Farmaceut, Lab Inovacao Tecnol Desenvolvimento Farmacos & Co, BR-87020900 Maringa, Parana, Brazil

[2] Univ Estadual Londrina, Programa Posgrad Microbiol, BR-86051990 Londrina, Parana, Brazil

[3] Univ Estadual Maringa, Dept Ciencias Basicas Saude, BR-87020900 Maringa, Parana, Brazil

[4] Univ Fed Fluminense, Dept Biol Marinha, BR-24001970 Rio De Janeiro, Brazil

[5] Univ Fed Fluminense, Programa Posgrad Quim Organ, BR-24020150 Rio De Janeiro, Brazil

来源：

PARASITOLOGY | 2010年 / 137卷 / 11期

关键词：

Trypanosoma cruzi; Laurencia dendroidea; elatol; anti-trypanosomal activity; mitochondrion; MARINE-ALGAE EXTRACTS; TRYPANOSOMA-CRUZI; CHAGAS-DISEASE; HALOGENATED METABOLITES; ANTIBACTERIAL ACTIVITY; ANTIPROTOZOAL ACTIVITY; BETA-LAPACHONE; OBTUSA; SESQUITERPENES; RHODOMELACEAE;

D O I：

10.1017/S003118201000034X

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Chagas' disease is a debilitating but comparatively neglected illness that affects about 15 million people. There is an urgent need to develop new, more effective, and less- toxic compounds. In this study, we assessed the in vitro anti- trypanosomal activity of the sesquiterpene elatol from the Brazilian red seaweed Laurencia dendroidea. We used electron microscopy to evaluate the effect of elatol on the morphology and ultrastructure of the parasite. Elatol showed a dose- dependent effect against the epimastigote, trypomastigote, and amastigote forms, with IC50 values of 45.4, 1.38, and 1.01 mu M, respectively. Observation of treated intracellular amastigotes by light microscopy demonstrated a total elimination of the infection at a dose of 3.0 mu M. In addition, the compound did not affect the red blood cells, and the CC50 value for LLCMK2 cells was 27.0 mu M. Transmission and scanning electron micrographs showed aberrant- shaped cells and breaks in the plasma membrane, prominent swollen mitochondria, and extensive formation of cytoplasmic vacuoles in all the forms. This is the first report of the anti- trypanosomal effect of the sesquiterpene elatol.

引用

页码：1661 / 1670

页数：10

共 47 条

[1] Extracts and sesquiterpene derivatives from the red alga Laurencia chondrioides with antibacterial activity against fish and human pathogenic bacteria [J].