Junctional Adhesion Molecule-A Promotes Proliferation and Inhibits Apoptosis of Gastric Cancer

被引：28

作者：

Ikeo, Koichi ^{[1
]}

Oshima, Tadayuki ^{[1
]}

Shan, Jing ^{[1
,2
]}

Matsui, Hirofumi ^{[3
]}

Tomita, Toshihiko ^{[1
]}

Fukui, Hirokazu ^{[1
]}

Watari, Jiro ^{[1
]}

Miwa, Hiroto ^{[1
]}

机构：

[1] Hyogo Coll Med, Dept Internal Med, Div Gastroenterol, Nishinomiya, Hyogo 6638501, Japan

[2] Third Peoples Hosp Chengdu, Dept Gastroenterol, Chengdu, Peoples R China

[3] Univ Tsukuba, Fac Med, Tsukuba, Ibaraki 305, Japan

来源：

HEPATO-GASTROENTEROLOGY | 2015年 / 62卷 / 138期

关键词：

Gastric cancer; Tight junctions; Proliferation; JAM-A; Bcl-xL; CELL INVASION; MONOCLONAL-ANTIBODY; DOWN-REGULATION; EXPRESSION; PERMEABILITY; MODULATION; CLAUDIN-3; LINE;

D O I：

10.5754/hge14919

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: Junctional adhesion molecules (JAMs) are known as integral constituents of cellular tight junctions. However, the functions of JAMS in Cancer tissues are controversial and the function of JAM-A in gastric cancer is unclear. Acordingly, we investigated the function of JAM-A in gastric epithelial and gastric cancer cell proliferation, invasion and apoptosis. Methodology: A normal rat gastric mucosa-derived cell line (RGM1), a rat gastric cancer-like cell line established from RGM1 (RGK1), and a human gastric cancer cell line (NCI-N87) were used in this study. To examine the expression of junctional proteins, immunoblotting and immunofluorescent staining were performed with specific antibodies (JAM-A, claudins, occludin and ZO-1). JAM-A was knocked down by small interfering RNA. Results: RGM1 and RGK1 expressed JAM-A, occludin and ZO-1 but not claudins. RGK1 were significantly more invasive than RGM1. JAM-A knockdown significantly decreased the proliferation and the invasion of RGK1 but not of RGM1. JAM-A knockdown significantly decreased the proliferation of NCI-N87 cells and significantly decreased expression of the anti-apoptotic protein Bcl-xL but not the expression of AKT or Mcl-1. Conclusions: JAM-A promotes proliferation and inhibits apoptosis of gastric cancer, suggesting that it has a pivotal role in gastric cancer progression.

引用

页码：540 / 545

页数：6

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