Protocol for Dissection and Dissociation of Zebrafish Telencephalon for Single-Cell Sequencing

被引：8

作者：

Cosacak, Mehmet Ilyas ^{[1
]}

Bhattarai, Prabesh ^{[1
]}

Kizil, Caghan ^{[1
,2
]}

机构：

[1] Helmholtz Assoc, German Ctr Neurodegenerat Dis DZNE Dresden, Tatzberg 41, D-01307 Dresden, Germany

[2] Tech Univ Dresden, Ctr Mol & Cellular Bioengn CMCB, Fetscherstr 105, D-01307 Dresden, Germany

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 01期

关键词：

D O I：

10.1016/j.xpro.2020.100042

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular sig-natures of cell types in a complex mixture of cells. A critical step in sc-Seq is pre-paring a single-cell suspension with a high number of viable cells. Here, we show how to dissect zebrafish telencephalon and how to dissociate it into a single-cell suspension. This is followed by flow cytometry-based sorting to enrich for neural progenitor stem cells. Our technique typically yields 70,000 live cells from one zebrafish telencephalon.For complete details on the use and execution of this protocol, please refer to Cosacak et al. (2019).

引用

页数：9

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