Effect of efferocytosis of apoptotic mesenchymal stem cells (MSCs) on C57BL/6 peritoneal macrophages function

被引:38
作者
Piraghaj, Maryam Ghahremani [1 ]
Soudi, Sara [2 ]
Ghanbarian, Hossein [3 ,4 ,5 ]
Bolandi, Zohreh [4 ]
Namaki, Saeed [1 ]
Hashemi, Seyed Mahmoud [1 ,3 ]
机构
[1] Shahid Beheshti Univ Med Sci, Sch Med, Dept Immunol, Tehran, Iran
[2] Tarbiat Modares Univ, Dept Immunol, Fac Med Sci, Tehran, Iran
[3] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Appl Cell Sci, Tehran, Iran
[4] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran
[5] Shahid Beheshti Univ Med Sci, Cellular & Mol Biol Res Ctr, Tehran, Iran
关键词
Efferocytosis; Apoptotic cells; Macrophages; Mesenchymal stem cells; IMMUNE-RESPONSES; STROMAL CELLS; PHAGOCYTOSIS; POLARIZATION; INFLAMMATION; ACTIVATION; RESOLUTION; CLEARANCE; SYSTEM; BALB/C;
D O I
10.1016/j.lfs.2018.09.052
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Phagocytic clearance of apoptotic cells (Efferocytosis) could affect the polarization of macrophages and promote M2 anti-inflammatory and regulatory phenotype and function. Here we tested the hypothesis that efferocytosis of apoptotic Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) promotes macrophage M2 polarization. In this study, Macrophages were incubated with apoptotic MSCs and after 48 h interleukin-10 (IL-10), transforming growth factor-alpha (TNF alpha), and nitric oxide (NO) production were measured. Furthermore, phagocytosis ability and arginase activity were analyzed. The results showed that apoptotic MSCs could reduce TNFa and NO production, and increase IL-10 levels. Moreover, arginase activity and phagocytosis ability were also increased in tested macrophages compared to controls. In Conclusion efferocytosis of AD-MSCs can alter the macrophages phenotype toward regulatory and anti-inflammatory phenotype.
引用
收藏
页码:203 / 212
页数:10
相关论文
共 36 条
  • [1] Phagocytosis of apoptotic cells in homeostasis
    Arandjelovic, Sanja
    Ravichandran, Kodi S.
    [J]. NATURE IMMUNOLOGY, 2015, 16 (09) : 907 - 917
  • [2] Mesenchymal stem cells alter macrophage immune responses to Leishmania major infection in both susceptible and resistance mice
    Dameshghi, Safura
    Zavaran-Hosseini, Ahmad
    Soudi, Sara
    Shirazi, Fatemeh Jalali
    Nojehdehi, Shahrzad
    Hashemi, Seyed Mahmoud
    [J]. IMMUNOLOGY LETTERS, 2016, 170 : 15 - 26
  • [3] Biochemical and functional characterization of three activated macrophage populations
    Edwards, Justin P.
    Zhang, Xia
    Frauwirth, Kenneth A.
    Mosser, David M.
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2006, 80 (06) : 1298 - 1307
  • [4] FADOK VA, 1992, J IMMUNOL, V148, P2207
  • [5] Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-β, PGE2, and PAF
    Fadok, VA
    Bratton, DL
    Konowal, A
    Freed, PW
    Westcott, JY
    Henson, PM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (04) : 890 - 898
  • [6] Clearance of Apoptotic Cells by Macrophages Induces Regulatory Phenotype and Involves Stimulation of CD36 and Platelet-Activating Factor Receptor
    Ferracini, Matheus
    Rios, Francisco J. O.
    Pecenin, Mateus
    Jancar, Sonia
    [J]. MEDIATORS OF INFLAMMATION, 2013, 2013
  • [7] Proinflammatory Clearance of Apoptotic Neutrophils Induces an IL-12lowIL-10high Regulatory Phenotype in Macrophages
    Filardy, Alessandra A.
    Pires, Dayana R.
    Nunes, Marise P.
    Takiya, Christina M.
    Freire-de-Lima, Celio G.
    Ribeiro-Gomes, Flavia L.
    DosReis, George A.
    [J]. JOURNAL OF IMMUNOLOGY, 2010, 185 (04) : 2044 - 2050
  • [8] Apoptotic cells, through transforming growth factor-β, coordinately induce anti-inflammatory and suppress pro-inflammatory eicosanoid and NO synthesis in murine macrophages
    Freire-de-Lima, Celio G.
    Xiao, Yi Qun
    Gardai, Shyra J.
    Bratton, Donna L.
    Schiemann, William P.
    Henson, Peter M.
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (50) : 38376 - 38384
  • [9] Mesenchymal Stromal Cells: Clinical Challenges and Therapeutic Opportunities
    Galipeau, Jacques
    Sensebe, Luc
    [J]. CELL STEM CELL, 2018, 22 (06) : 824 - 833
  • [10] Alternative activation of macrophages
    Gordon, S
    [J]. NATURE REVIEWS IMMUNOLOGY, 2003, 3 (01) : 23 - 35