Metal phosphonates applied to biotechnologies:: A novel approach to oligonucleotide microarrays

被引:86
作者
Bujoli, B
Lane, SM
Nonglaton, G
Pipelier, M
Léger, J
Talham, DR
Tellier, C
机构
[1] CNRS, UMR 6513, Organ Synth Lab, F-44322 Nantes, France
[2] CNRS, FR 2465, F-44322 Nantes, France
[3] Univ Florida, Dept Chem, Gainesville, FL 32611 USA
[4] UFR Med Physiol, INSERM, U 533, F-44035 Nantes, France
[5] CNRS, UMR 6204, Lab Biotechnol Biocatalyse Bioregulat, F-44322 Nantes, France
关键词
DNA; microarrays; monolayers; oligonucleotides; phosphonates;
D O I
10.1002/chem.200400960
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A new process for preparing, oligonucleotide arrays is described that uses surface grafting chemistry which is fundamentally different from the electrostatic adsorption and organic covalent binding methods normally employed. Solid supports are modified with a mixed organic/inorganic zirconium phosphonate monolayer film providing a stable, well-defined interface. Oligonucleotide probes terminated with phosphate are spotted directly on to the zirconated surface forming a covalent linkage. Specific binding of terminal phosphate groups with minimal binding of the internal phosphate diesters has been demonstrated. ne mixed organic/inorganic thin films have also been extended for use arraying DNA duplex probes, and therefore represent a viable general approach to DNA-based bioarrays. Ideas for interfacing mixed organic/inorganic interfaces to other bioapplications are also discussed.
引用
收藏
页码:1981 / 1988
页数:8
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