Application of Nanoscale Poly(divinyl-benzene vinylpyrrolidone) as Solid-phase Extraction Adsorbent prior to LC-MS-MS for the Determination of Paralytic Shellfish Poisoning Toxins in Shellfish

被引：0

作者：

Zhang Xiaoling ^{[1
]}

Feng Bing ^{[1
]}

Cai Youqiong ^{[1
]}

Yu Huijuan ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Minstry Agriculgure China, Supervis & Test Ctr Aquat Producs, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

来源：

MATERIALS, MECHATRONICS AND AUTOMATION, PTS 1-3 | 2011年 / 467-469卷

关键词：

Paralytic shellfish poisoning toxins; Solid-phase extraction; LC-MS-M; Nanoscale adsorbent; Shellfish; PERFORMANCE LIQUID-CHROMATOGRAPHY; INDUCED FLUORESCENCE DETECTION;

D O I：

10.4028/www.scientific.net/KEM.467-469.2085

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

In the present study, the feasibility of the poly(divinyl-benzene vinylpyrrolidone) as solid-phase extraction (SPE) nanoscale adsorbent prior to LC-MS-MS method for the sensitive and selective determination of eight paralytic shellfish poisoning (PSP) (GTX1, GTX2, GTX3, GTX4, GTX5, dcGTX2, dcGTX3 and dcNEO-b) toxins in the shellfish was investigated. Various parameters affecting SPE efficiency including the eluent and its volume, and sample volume were studied. The application of the SPE can remove most of the ematrix interference compared with the conventional SPE procedure. By using slected reaction monitoring (SRM) mode in LC-MS-MS analysis, the acquired calibration curves were strictly linear (r(2) > 0.996) for the eight targeted analyses. The feasibility of the proposed method was finally validated by quantifing the eight PSPs in the shellfish samples (scallop, Crassostrea gigas and Ruditapes philippinarum) with the satisfitory recoveries of more than 80% with RSDs less than 10%.

引用

页码：2085 / 2090

页数：6

共 15 条

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