Central effects of long-term relaxin expression in the rat

被引：4
作者：
Silvertown, JD
Fraser, R
Poterski, RS
Geddes, B
Summerlee, AJS
机构：
[1] Univ Guelph, Ontario Vet Coll, Dept Biomed Sci, Guelph, ON N1G 2W1, Canada
[2] Univ Hlth Network, Ontario Canc Inst, Div Expt Therapeut, Toronto, ON M5G 2M1, Canada
[3] Elixir Pharmaceut Inc, Cambridge, MA 02139 USA
来源：
RELAXIN AND RELATED PEPTIDES: FOURTH INTERNATIONAL CONFERENCE | 2005年 / 1041卷
关键词：
relaxin; adenovirus; lateral ventricle; vasopressin; plasma osmolality; FIBROSIS; ADENOVIRUS; RECEPTORS; HORMONE; CELLS;
D O I：
10.1196/annals.1282.033
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A recombinant adenovirus containing the human H2 preprorelaxin (hH2) cDNA and a reporter gene was coinjected with a transactivator virus (Ad-tTA) into the lateral cerebral ventricles of female rats. Cardiovascular effects were measured over a 21-day period. Circulating vasopressin in the periphery was significantly greater (P <.0001) in the relaxin-treated group throughout the experimental period, compared with controls. There was a significant decrease in plasma osmolality (P <.05) by approximately 10 mmol/L in the treated group by day 14. Immunofluorescence for hH2 present in cryosections showed rAd transduction and hH2 expression from ependymal cells of the ventricular system. Adenovirus-mediated delivery of hH2 to the brain is capable of producing bioactive relaxin that affects cardiovascular parameters.
引用
收藏
页码：216 / 222
页数：7
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