In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami

被引：20

作者：

Wu, Na ^{[1
]}

Zhou, Xingfei ^{[2
]}

Czajkowsky, Daniel M. ^{[1
]}

Ye, Ming ^{[1
]}

Zeng, Dongdong ^{[1
]}

Fu, Yanming ^{[1
]}

Fan, Chunhai ^{[1
]}

Hu, Jun ^{[1
]}

Li, Bin ^{[1
]}

机构：

[1] Chinese Acad Sci, Lab Phys Biol, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

[2] Ningbo Univ, Dept Phys, Ningbo 315211, Zhejiang, Peoples R China

来源：

NANOSCALE | 2011年 / 3卷 / 06期

关键词：

REAL-TIME; BIOTIN BINDING; STREPTAVIDIN; PROTEIN; KINETICS; PLATFORM; AVIDIN; NANOARRAYS; BILAYERS; CRYSTALS;

D O I：

10.1039/c1nr10181a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Individual biomolecular binding events were recorded in situ by combining time-lapse atomic force microscopy and DNA origami. Single streptavidin molecules bound to specifically biotinyated DNA origami were simply counted as a function of time to obtain a direct measure of the binding rate.

引用

页码：2481 / 2484

页数：4

共 54 条

[1] Programmable Periodicity of Quantum Dot Arrays with DNA Origami Nanotubes [J].

Bui, Hieu ;

Onodera, Craig ;

Kidwell, Carson ;

Tan, YerPeng ;

Graugnard, Elton ;

Kuang, Wan ;

Lee, Jeunghoon ;

Knowlton, William B. ;

Yurke, Bernard ;

Hughes, William L. .

NANO LETTERS, 2010, 10 (09) :3367-3372

[2] Scanning force microscopy under aqueous solutions [J].

Bustamante, C ;

Rivetti, C ;

Keller, DJ .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1997, 7 (05) :709-716

[3] Self-assembled DNA networks and their electrical conductivity [J].

Cai, LT ;

Tabata, H ;

Kawai, T .

APPLIED PHYSICS LETTERS, 2000, 77 (19) :3105-3106

[4] Spatially addressable multiprotein nanoarrays templated by aptamer-tagged DNA nanoarchitectures [J].

Chhabra, Rahul ;

Sharma, Jaswinder ;

Ke, Yonggang ;

Liu, Yan ;

Rinker, Sherri ;

Lindsay, Stuart ;

Yan, Hao .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (34) :10304-+

[5] Rapid prototyping of 3D DNA-origami shapes with caDNAno [J].

Douglas, Shawn M. ;

Marblestone, Adam H. ;

Teerapittayanon, Surat ;

Vazquez, Alejandro ;

Church, George M. ;

Shih, William M. .

NUCLEIC ACIDS RESEARCH, 2009, 37 (15) :5001-5006

[6] IMAGING CRYSTALS, POLYMERS, AND PROCESSES IN WATER WITH THE ATOMIC FORCE MICROSCOPE [J].

DRAKE, B ;

PRATER, CB ;

WEISENHORN, AL ;

GOULD, SAC ;

ALBRECHT, TR ;

QUATE, CF ;

CANNELL, DS ;

HANSMA, HG ;

HANSMA, PK .

SCIENCE, 1989, 243 (4898) :1586-1589

[7] Controlled Delivery of DNA Origami on Patterned Surfaces [J].

Gerdon, Aren E. ;

Oh, Seung Soo ;

Hsieh, Kuangwen ;

Ke, Yonggang ;

Yan, Hao ;

Soh, H. Tom .

SMALL, 2009, 5 (17) :1942-1946

[8]

GREEN NM, 1990, METHOD ENZYMOL, V184, P51

[9] APPLICATIONS FOR ATOMIC-FORCE MICROSCOPY OF DNA [J].

HANSMA, HG ;

LANEY, DE ;

BEZANILLA, M ;

SINSHEIMER, RL ;

HANSMA, PK .

BIOPHYSICAL JOURNAL, 1995, 68 (05) :1672-1677

[10] TAPPING MODE ATOMIC-FORCE MICROSCOPY IN LIQUIDS [J].

HANSMA, PK ;

CLEVELAND, JP ;

RADMACHER, M ;

WALTERS, DA ;

HILLNER, PE ;

BEZANILLA, M ;

FRITZ, M ;

VIE, D ;

HANSMA, HG ;

PRATER, CB ;

MASSIE, J ;

FUKUNAGA, L ;

GURLEY, J ;

ELINGS, V .

APPLIED PHYSICS LETTERS, 1994, 64 (13) :1738-1740

← 1 2 3 4 5 6 →