1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein

被引：3

作者：

Liu, Jiajing ^{[1
]}

Du, Zhenming ^{[1
]}

Albracht, Clayton D. ^{[1
]}

Naidu, Roshni O. ^{[1
]}

Mills, Kenneth V. ^{[2
]}

Wang, Chunyu ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Biol, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[2] Coll Holy Cross, Dept Chem, Worcester, MA 01610 USA

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2011年 / 5卷 / 02期

基金：

美国国家科学基金会;

关键词：

Intein; NMR; Assignment; Hyperthermophile; DNA polymerase II; Pyrococcus abyssi; PROTEIN; GLUTAMINE;

D O I：

10.1007/s12104-011-9307-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Protein splicing is a precise post-translational process mediated by inteins. Inteins are intervening proteins that cleave themselves from a precursor protein while joining the flanking sequences. Here we report the N-15, C-13, and H-1 chemical shift assignments of the intein from DNA polymerase II of Pyrococcus abyssi (Pab PolII intein), which has been recombinantly overexpressed and isotopically labeled. The NMR assignments of Pab PolII intein are essential for solution structure determination and protein dynamics study.

引用

页码：233 / 235

页数：3

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