Photoelectrochemical competitive DNA hybridization assay using semiconductor quantum dot conjugated oligonucleotides

被引：16

作者：

Bas, Deniz ^{[1
]}

Boyaci, Ismail Hakki ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Food Engn, TR-06800 Ankara, Turkey

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2011年 / 400卷 / 03期

关键词：

Photoelectrochemistry; DNA; Quantum dots; Genosensor; Photocurrent; METHYLENE-BLUE; ELECTRODES; GOLD; ELECTROCHEMILUMINESCENCE;

D O I：

10.1007/s00216-011-4827-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A competitive DNA hybridization assay based on the photoelectrochemistry of the semiconductor quantum dot-single stranded DNA conjugates (QD-ssDNA) was developed. Hybridization of QD-ssDNA with the capture probe DNA immobilized on the indium-tin oxide electrodes enables photocurrent generation when the electrochemical cell was illuminated with a light source. Upon the competition between QD-ssDNA and single-stranded target DNA, the photocurrent response decreased with the increase in the target DNA concentration. A linear relationship between the photocurrent and the target DNA concentration was obtained (R (2) = 0.991). The selectivity of system towards the target DNA was also demonstrated using non-complementary sample.

引用

页码：703 / 707

页数：5