Interaction of peptides with graphene oxide and its application for real-time monitoring of protease activity

被引：200

作者：

Zhang, Min ^{[1
]}

Yin, Bin-Cheng ^{[1
]}

Wang, Xian-Fei ^{[1
]}

Ye, Bang-Ce ^{[1
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 08期

关键词：

RESONANCE ENERGY-TRANSFER; PLATFORM; THROMBIN;

D O I：

10.1039/c0cc04887a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have developed a novel graphene-based biosensing platform using peptides as probe biomolecules, and demonstrated its feasibility in the application of real-time monitoring of protease activity based on FRET between GO and dye-labeled peptides. This assay allows the rapid and accurate determination of enzyme kinetic parameters as well as inhibition constants.

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收藏

页码：2399 / 2401

页数：3

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