Up-converting phosphor technology-based lateral flow assay for detection of Schistosoma circulating anodic antigen in serum

被引：165

作者：

Corstjens, Paul L. A. M. ^{[1
]}

van Lieshout, Lisette ^{[2
]}

Zuiderwijk, Michel ^{[1
]}

Kornelis, Dieuwke ^{[2
]}

Tanke, Hans J. ^{[1
]}

Deelder, Andre M. ^{[2
]}

van Dam, Govert J. ^{[2
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Mol Cell Biol, NL-2300 RC Leiden, Netherlands

[2] Leiden Univ, Med Ctr, Dept Parasitol, NL-2300 RC Leiden, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 01期

关键词：

D O I：

10.1128/JCM.00877-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Schistosoma sp. circular anodic antigen (CAA) serum concentrations reflect actual worm burden in a patient and are a valuable tool for population screening and epidemiological research. However, for the diagnosis of individual imported schistosomiasis cases, the current enzyme-linked immunosorbent assay (ELISA) lacks sensitivity and robustness. Therefore, a lateral flow (LF) assay was developed to test CAA in serum for individual diagnosis of imported active schistosome infections. Application of fluorescent submicron-sized up-converting phosphor technology (UPT) reporter particles increased analytical sensitivity compared to that of the standard ELISA method. Evaluation of the UPT-LF test with a selection of 40 characterized epidemiologic samples indicated a good correlation between signal intensity and infection intensity. Subsequently, the UPT-LF assay was applied to 166 serum samples of Dutch residents (immigrants and travelers) suspected of schistosomiasis, a case in which group routine antibody detection frequently fails straightforward diagnosis. The UPT-LF assay identified 36 CAA-positive samples, compared to 15 detected by CAA-ELISA. In conclusion, the UPT-LF assay is a low-complexity test with higher sensitivity than the CAA-ELISA, well suited for laboratory diagnosis of individual active Schistosoma infections.

引用

页码：171 / 176

页数：6

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