miR-1307-3p suppresses the chondrogenic differentiation of human adipose-derived stem cells by targeting BMPR2

被引:11
作者
Yang, Zhen [1 ,2 ]
Li, Rui [2 ]
Ao, Jun [3 ]
Wa, Qing-De [3 ]
Zhang, Yi [2 ]
Chen, Long [2 ]
Wen, Jing [2 ]
Chen, Biao [2 ]
Pan, Wei [2 ]
Li, Bo [2 ]
Tian, Xiao-Bin [1 ,2 ]
机构
[1] Guizhou Univ, Coll Med, 4 Beijing Rd, Guiyang 550025, Guizhou, Peoples R China
[2] Guizhou Univ, Peoples Hosp, Guizhou Prov Peoples Hosp, 83 Zhongshan East Rd, Guiyang 550002, Guizhou, Peoples R China
[3] Zunyi Med Coll, Affiliated Hosp, Zunyi 563000, Guizhou, Peoples R China
基金
中国国家自然科学基金;
关键词
microRNA-1307-3p; human adipose-derived stem cells; chondrogenic differentiation; bone morphogenetic protein receptor type 2; mothers against decapentaplegic homolog 1/5/8; OSTEOGENIC DIFFERENTIATION; EXPRESSION; RECEPTOR; MICRORNAS; OSTEOARTHRITIS; TISSUE; GENE;
D O I
10.3892/ijmm.2018.3891
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
MicroRNAs (miRs) are involved in several physiological processes, including chondrogenic differentiation, however, their expression and roles in the chondrogenic differentiation of human adipose-derived stem cells (hADSCs) remain to be fully elucidated to date. Our previous study showed that miR-1307-3p was significantly downregulated during chondrogenic differentiation by microarray and northern blot analysis. The present study aimed to investigate the effects of miR-1307-3p on chondrogenic differentiation and the underlying mechanisms. First, the decreased expression of miR-1307-3p was confirmed by reverse transcription-quantitative polymerase chain reaction analysis. Subsequently, gain- and loss-of-function of miR-1307-3p experiments showed that the overexpression of miR-1307-3p suppressed the deposition of cartilage matrix proteoglycans and decreased the expression of cartilage-related markers, including sex determining region Y-box 9, collagen type II 1 chain and aggrecan, whereas the knockdown of miR-1307-3p had the opposite effect. In addition, bone morphogenetic protein receptor type 2 (BMPR2) was identified as a target of miR-1307-3p. Further mechanistic investigations showed that miR-1307-3p attenuated the chondrogenic differentiation of hADSCs at least partly by inhibiting BMPR2-mothers against decapentaplegic signaling pathways. In conclusion, the findings revealed that miR-1307-3p inhibited the chondrogenic differentiation of hADSCs by targeting BMPR2 and its down-stream signaling pathway, which may provide novel therapeutic clues for the treatment of cartilage injury.
引用
收藏
页码:3115 / 3124
页数:10
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