Fluorescence Microscopy of the HIV-1 Envelope

被引：7

作者：

Carravilla, Pablo ^{[1
,2
,3
,4
]}

Nieva, Jose L. ^{[3
,4
]}

Eggeling, Christian ^{[1
,2
,5
,6
]}

机构：

[1] Friedrich Schiller Univ Jena, Inst Appl Opt & Biophys, Max Wien Pl 1, D-07743 Jena, Germany

[2] Leibniz Inst Photon Technol, Albert Einstein Str 9, D-07743 Jena, Germany

[3] Univ Basque Country, Dept Biochem & Mol Biol, UPV EHU, POB 644, Bilbao 48080, Spain

[4] Univ Basque Country, UPV EHU, CSIC, Inst Biofis, E-48940 Leioa, Spain

[5] Univ Oxford, MRC Weatherall Inst Mol Med, Headley Way, Oxford OX3 9DS, England

[6] Friedrich Schiller Univ Jena, Jena Ctr Soft Matter, Philosophenweg 7, D-07743 Jena, Germany

来源：

VIRUSES-BASEL | 2020年 / 12卷 / 03期

基金：

英国惠康基金; 英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

microscopy; HIV; envelope; lipid; membrane; Env; fluorescence; super-resolution; HUMAN-IMMUNODEFICIENCY-VIRUS; VIRION-ASSOCIATED CHOLESTEROL; PLASMA-MEMBRANE; IN-VITRO; GAG; PROTEIN; TYPE-1; ENV; SURFACE; FUSION;

D O I：

10.3390/v12030348

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human immunodeficiency virus (HIV) infection constitutes a major health and social issue worldwide. HIV infects cells by fusing its envelope with the target cell plasma membrane. This process is mediated by the viral Env glycoprotein and depends on the envelope lipid composition. Fluorescent microscopy has been employed to investigate the envelope properties, and the processes of viral assembly and fusion, but the application of this technique to the study of HIV is still limited by a number of factors, such as the small size of HIV virions or the difficulty to label the envelope components. Here, we review fluorescence imaging studies of the envelope lipids and proteins, focusing on labelling strategies and model systems.

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页数：12

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