Enoate reductases of Clostridia -: Cloning, sequencing, and expression

被引：43

作者：

Rohdich, F ^{[1
]}

Wiese, A ^{[1
]}

Feicht, R ^{[1
]}

Simon, H ^{[1
]}

Bacher, A ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, Inst Organ Chem & Biochem, D-85747 Garching, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 08期

关键词：

D O I：

10.1074/jbc.M008656200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enr genes specifying enoate reductases of Clos tridium tyrobutyricum and Clostridium thermoaceticum were cloned and sequenced. Sequence comparison A shows that enoate reductases are similar to a family of flavoproteins comprising 2,4-dienoyl-coenzyme A reductase from Escherichia coli and old yellow enzyme from yeast. The C. thermoaceticum enr gene product was expressed in recombinant Escherichia coli cells growing under anaerobic conditions. The recombinant enzyme was purified and characterized.

引用

页码：5779 / 5787

页数：9

共 48 条

[1] THE REDUCTION OF ALLYL ALCOHOLS BY CLOSTRIDIUM SPECIES IS CATALYZED BY THE COMBINED ACTION OF ALCOHOL-DEHYDROGENASE AND ENOATE REDUCTASE [J].