Use of conventional or non-conventional treatments of biochar for lipase immobilization

被引：32

作者：

Almeida, Lays C. ^{[1
]}

Barbosa, Anderson S. ^{[1
]}

Fricks, Alini T. ^{[1
]}

Freitas, Lisiane S. ^{[2
]}

Lima, Alvaro S. ^{[1
]}

Soares, Cleide M. F. ^{[1
]}

机构：

[1] Univ Tiradentes, Inst Technol & Res, Ave Murilo Dantas 300, BR-49032490 Aracaju, Sergipe, Brazil

[2] Univ Fed Sergipe, Dept Chem, Av Marechal Rondon,S-N, BR-49100000 Sao Cristovelo, Sergipe, Brazil

来源：

PROCESS BIOCHEMISTRY | 2017年 / 61卷

关键词：

Biochar; Conventional treatment (muffle) and non-conventional (ultrasound; microwave); Immobilization; Lipase; MESOPOROUS ACTIVATED CARBON; K2CO3; ACTIVATION; BIOCATALYSIS; ADSORPTION; RESIDUE; STRAW; PEEL; OIL; KOH;

D O I：

10.1016/j.procbio.2017.06.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this work was to evaluate the influence of dichloromethane (CH2Cl2), potassium hydroxide (KOH) and phosphoric acid (H3PO4) in the treatment of biochar from guava seeds on conventional and non conventional techniques (ultrasound and microwave) for the immobilization of Burkholderia cepacia lipase (BCL) by physical adsorption. The effects of the different treatments on the physical and chemical properties of the biochar were evaluated by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The immobilization of the BCL on the biochar was evaluated in hydrolysis reactions of olive oil. The conventional treatment of the biochar with KOH showed increased modification on the surface of the biochar, which presented a highly porous surface, and a greater activity for the immobilized biocatalyst compared with the other treatments. The results revealed the potential of biochar as a support novelty for the immobilization of enzymes and for their application in biocatalysis.

引用

页码：124 / 129

页数：6

共 27 条

[1]

Ademiluyi F. T., 2009, Journal of Applied Sciences and Environmental Management, V13, P39

[2] Industrial applications of enzyme biocatalysis: Current status and future aspects [J].

Choi, Jung-Min ;

Han, Sang-Soo ;

Kim, Hak-Sung .

BIOTECHNOLOGY ADVANCES, 2015, 33 (07) :1443-1454

[3] Adsorptive removal of chloramphenicol from wastewater by NaOH modified bamboo charcoal [J].

Fan, Ye ;

Wang, Bin ;

Yuan, Songhu ;

Wu, Xiaohui ;

Chen, Jing ;

Wang, Linling .

BIORESOURCE TECHNOLOGY, 2010, 101 (19) :7661-7664

[4] Potential of jackfruit peel as precursor for activated carbon prepared by microwave induced NaOH activation [J].

Foo, K. Y. ;

Hameed, B. H. .

BIORESOURCE TECHNOLOGY, 2012, 112 :143-150

[5] Mesoporous activated carbon from wood sawdust by K2CO3 activation using microwave heating [J].

Foo, K. Y. ;

Hameed, B. H. .

BIORESOURCE TECHNOLOGY, 2012, 111 :425-432

[6] Coconut husk derived activated carbon via microwave induced activation: Effects of activation agents, preparation parameters and adsorption performance [J].

Foo, K. Y. ;

Hameed, B. H. .

CHEMICAL ENGINEERING JOURNAL, 2012, 184 :57-65

[7] Porous structure and adsorptive properties of pineapple peel based activated carbons prepared via microwave assisted KOH and K2CO3 activation [J].

Foo, K. Y. ;

Hameed, B. H. .

MICROPOROUS AND MESOPOROUS MATERIALS, 2012, 148 (01) :191-195

[8] Preparation and characterization of activated carbon from sunflower seed oil residue via microwave assisted K2CO3 activation [J].

Foo, K. Y. ;

Hameed, B. H. .

BIORESOURCE TECHNOLOGY, 2011, 102 (20) :9794-9799

[9] Utilization of rice husks as a feedstock for preparation of activated carbon by microwave induced KOH and K2CO3 activation [J].

Foo, K. Y. ;

Hameed, B. H. .

BIORESOURCE TECHNOLOGY, 2011, 102 (20) :9814-9817

[10] Biochar Derived from Agricultural and Forestry Residual Biomass: Characterization and Potential Application for Enzymes Immobilization [J].

Gonzalez, M. E. ;

Cea, M. ;

Sangaletti, N. ;

Gonzalez, A. ;

Toro, C. ;

Diez, M. C. ;

Moreno, N. ;

Querol, X. ;

Navia, R. .

JOURNAL OF BIOBASED MATERIALS AND BIOENERGY, 2013, 7 (06) :724-732

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