Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

被引：50

作者：

Kourmouli, N

Sun, YM

van der Sar, S

Singh, PB ^{[1
]}

Brown, JP

机构：

[1] Forschungszentrum Borstel, Dept Immunol & Cell Biol, Div Tumor Biol, D-23845 Borstel, Germany

[2] Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England

[3] Univ Edinburgh, Wellcome Trust Ctr Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[4] Massachusetts Gen Hosp, Cutaneous Biol Res Ctr, Charlestown, MA 02129 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 337卷 / 03期

关键词：

HP1; histone code; heterochromatin; epigenetics;

D O I：

10.1016/j.bbrc.2005.09.132

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9143) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it." (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：901 / 907

页数：7

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