Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

被引：50
作者：
Kourmouli, N
Sun, YM
van der Sar, S
Singh, PB [1 ]
Brown, JP
机构：
[1] Forschungszentrum Borstel, Dept Immunol & Cell Biol, Div Tumor Biol, D-23845 Borstel, Germany
[2] Univ Leeds, Sch Biochem & Mol Biol, Leeds LS2 9JT, W Yorkshire, England
[3] Univ Edinburgh, Wellcome Trust Ctr Cell & Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland
[4] Massachusetts Gen Hosp, Cutaneous Biol Res Ctr, Charlestown, MA 02129 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 337卷 / 03期
关键词：
HP1; histone code; heterochromatin; epigenetics;
D O I：
10.1016/j.bbrc.2005.09.132
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9143) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only "deciphers" the histone code but can also "encode it." (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：901 / 907
页数：7
相关论文
共 33 条
[1]   Regulated recruitment of HP1 to a euchromatic gene induces mitotically heritable, epigenetic gene silencing: a mammalian cell culture model of gene variegation [J].
Ayyanathan, K ;
Lechner, MS ;
Bell, P ;
Maul, GG ;
Schultz, DC ;
Yamada, Y ;
Tanaka, K ;
Torigoe, K ;
Rauscher, FJ .
GENES & DEVELOPMENT, 2003, 17 (15) :1855-1869
[2]   Structure of the chromatin binding (chromo) domain from mouse modifier protein 1 [J].
Ball, LJ ;
Murzina, NV ;
Broadhurst, RW ;
Raine, ARC ;
Archer, SJ ;
Stott, FJ ;
Murzin, AG ;
Singh, PB ;
Domaille, PJ ;
Laue, ED .
EMBO JOURNAL, 1997, 16 (09) :2473-2481
[3]   Maintenance of stable heterochromatin domains by dynamic HP1 binding [J].
Cheutin, T ;
McNairn, AJ ;
Jenuwein, T ;
Gilbert, DM ;
Singh, PB ;
Misteli, T .
SCIENCE, 2003, 299 (5607) :721-725
[4]   Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals [J].
Cowell, IG ;
Aucott, R ;
Mahadevaiah, SK ;
Burgoyne, PS ;
Huskisson, N ;
Bongiorni, S ;
Prantera, G ;
Fanti, L ;
Pimpinelli, S ;
Wu, R ;
Gilbert, DM ;
Shi, W ;
Fundele, R ;
Morrison, H ;
Jeppesen, P ;
Singh, PB .
CHROMOSOMA, 2002, 111 (01) :22-36
[5]   Heterochromatin protein 1 modifies mammalian PEV in a dose- and chromosomal-context-dependent manner [J].
Festenstein, R ;
Sharghi-Namini, S ;
Fox, M ;
Roderick, K ;
Tolaini, M ;
Norton, T ;
Saveliev, A ;
Kioussis, D ;
Singh, P .
NATURE GENETICS, 1999, 23 (04) :457-461
[6]   Mouse centric and pericentric satellite repeats form distinct functional heterochromatin [J].
Guenatri, M ;
Bailly, D ;
Maison, C ;
Almouzni, G .
JOURNAL OF CELL BIOLOGY, 2004, 166 (04) :493-505
[7]   Dynamic interaction of HMGA1a proteins with chromatin [J].
Harrer, M ;
Lührs, H ;
Bustin, M ;
Scheer, U ;
Hock, R .
JOURNAL OF CELL SCIENCE, 2004, 117 (16) :3459-3471
[8]   M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin [J].
Horsley, D ;
Hutchings, A ;
Butcher, GW ;
Singh, PB .
CYTOGENETICS AND CELL GENETICS, 1996, 73 (04) :308-311
[9]  
IZUMI M, 1999, J CELL BIOCHEM, P280
[10]   Specificity of the HP1 chromo domain for the methylated N-terminus of histone H3 [J].
Jacobs, SA ;
Taverna, SD ;
Zhang, YN ;
Briggs, SD ;
Li, JM ;
Eissenberg, JC ;
Allis, CD ;
Khorasanizadeh, S .
EMBO JOURNAL, 2001, 20 (18) :5232-5241
1234