Patient-Side Assay of Lipase Activity Correlating with Pancreatic Lipase Immunoreactivity in the Dog

被引：15

作者：

Ishioka, Katsumi ^{[1
]}

Hayakawa, Noriyuki ^{[2
]}

Nakamura, Kentaro ^{[3
]}

Terashima, Kaoru ^{[3
]}

机构：

[1] Nippon Vet & Life Sci Univ, Dept Vet Nursing, Sch Vet Nursing & Technol, Fac Vet Sci, Musashino, Tokyo 1808602, Japan

[2] Nippon Vet & Life Sci Univ, Vet Med Teaching Hosp, Musashino, Tokyo 1808602, Japan

[3] FUJIFILM Corp, Pharmaceut & Healthcare Res Labs, Res & Dev Management Headquarters, Kaisei, Kanagawa 2588577, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 11期

关键词：

dry chemistry test; lipase; pancreatitis; SERUM; VALIDATION; DIAGNOSIS;

D O I：

10.1292/jvms.11-0166

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Pancreatitis is a common exocrine pancreatic disease in dogs, and the pancreatic lipase immunoreactivity (PLI) test is used for diagnosis. Enzyme catalytic assay is thought to have low specificity, but a lipase activity assay with increased specificity has been developed in human clinical chemistry. We measured serum lipase activity of 65 client-owned dogs using the newly developed FUJI DRI-CHEM slide and compared the results with their PLI concentrations. The results showed a good correlation (r=0.91), and the normal and pancreatitis dogs identified based on the PLI values were correctly separated based on lipase activity. The present study suggests that FUJI DRI-CHEM lipase activity would be helpful for diagnosis Of pacreatitis in dogs and, in particular, that it can be used as a patient-side assay and contributes to immediate treatment.

引用

页码：1481 / 1483

页数：3

共 10 条

[1]

Baginsky M.L.BrownW.V, 1997, J LIPID RES, V18, P423

[2]

GARGOURI Y, 1983, J LIPID RES, V24, P1336

[3] HUMAN GASTRIC LIPASE - THE EFFECT OF AMPHIPHILES [J].