Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter

被引：37

作者：

Xu, Fangfang ^{[1
]}

Liao, Kangsheng ^{[1
]}

Wu, Yunshan ^{[1
]}

Pan, Qi ^{[1
]}

Wu, Lilan ^{[1
]}

Jiao, Hong ^{[2
]}

Guo, Dean ^{[1
,3
]}

Li, Ben ^{[1
]}

Liu, Bo ^{[1
]}

机构：

[1] Guangzhou Univ Chinese Med, Guangdong Prov Acad Chinese Med Sci, Affiliated Hosp 2, Guangzhou 510006, Guangdong, Peoples R China

[2] Guangdong Entry Exit Inspect & Quarantine Bur, Guangzhou 510006, Guangdong, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, 501 Haike Rd, Shanghai 201203, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2016年 / 151卷

基金：

中国国家自然科学基金;

关键词：

Borojoa sorbilis cuter; Polysaccharide; Structural characterization; Sulfation; Antitumor activity; STRUCTURAL-CHARACTERIZATION; ANTICOAGULANT ACTIVITY; BIOLOGICAL-ACTIVITIES; GANODERMA-ATRUM; GREEN-ALGA; ANTIOXIDANT; DERIVATIVES; EXTRACTION;

D O I：

10.1016/j.carbpol.2016.05.091

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Extraction optimization, purification, characterization, sulfation and antitumor activity of polysaccharides from the fruit body of Borojoa sorbilis cuter were investigated in present study. The optimal Ultrahigh Pressure extraction condition was determined as: extraction once with the solid-liquid ratio of 1:10 in 30 degrees C and 1500 Mpa for crude polysaccharide (BP) and experimental yield was 8.28%. Four water-soluble polysaccharides named as BP1-1, BP1-2, BP1-3 and BP1-4, with molecular weight of 35.8, 32.4, 30.1 and 27.7 kDa, were purified by DEAE Sepharose and Superdex 200 chromatography. On the basis of chemical and spectroscopic analyses, BP1-1-BP1-4 were found to be neutral beta-D-galactan containing a (1 -> 4) linked backbone. S-BP1s with the DSS of 1.18, was sulfated by chloro-sulfonic acid-pyridine method. Furthermore, S-BP1s exhibited significant in vitro antitumor activity against liver cancer HepG2 and lung cancer A549 cells in a dose-dependent manner. The results indicated that S-BP1s could be potentially developed as functional antitumor drug. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：364 / 372

页数：9

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