Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure

被引：85

作者：

Ge ZhiLei ^{[1
]}

Pei Hao ^{[1
]}

Wang LiHua ^{[1
]}

Song ShiPing ^{[1
]}

Fan ChunHai ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China

来源：

SCIENCE CHINA-CHEMISTRY | 2011年 / 54卷 / 08期

基金：

中国国家自然科学基金;

关键词：

DNA nanostructure; surface density; electrochemical; SEQUENCE-SPECIFIC DETECTION; INSOLUBLE PRODUCT; GOLD SURFACES; HYBRIDIZATION; PRECIPITATION; AMPLIFICATION; INTERROGATION; ELECTRODES; BIOSENSORS; PROBE;

D O I：

10.1007/s11426-011-4327-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.

引用

页码：1273 / 1276

页数：4

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