Utility of flow cytometry in canine primary cutaneous and matched nodal mast cell tumor

被引：10

作者：

Sulce, M. ^{[1
]}

Marconato, L. ^{[2
]}

Martano, M. ^{[1
]}

Iussich, S. ^{[1
]}

Dentini, A. ^{[3
]}

Melega, M. ^{[1
]}

Miniscalco, B. ^{[1
]}

Riondato, F. ^{[1
]}

机构：

[1] Univ Turin, Dept Vet Sci, Largo P Braccini 2, I-10095 Turin, Italy

[2] Ctr Oncol Vet, Via San Lorenzo 1-4, I-40037 Bologna, Italy

[3] Clin Vet Tyrus, Via Bartocci 1-G, I-05100 Terni, Italy

来源：

VETERINARY JOURNAL | 2018年 / 242卷

关键词：

Dog; Mast cell tumor; Lymph node; Flow cytometry; IMMUNOPHENOTYPIC ANALYSIS; SYSTEMIC MASTOCYTOSIS; DOGS; LINE; TIME; KIT; VALIDATION; EXPRESSION; GENERATION; RECEPTORS;

D O I：

10.1016/j.tvjl.2018.10.004

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Mast cell tumors (MCT) are among the most frequent tumors in dogs, but studies regarding canine mast cell immunophenotype are lacking. The aim of this study was to assess the feasibility of flow cytometric analysis of MCTs, to describe canine MCTs immunophenotype(s), and to evaluate the ability of flow cytometry to detect mast cells in lymph node aspirates. Thirty-four primary canine MCTs and 12 draining lymph nodes were evaluated regarding the expression of CD117, IgE, CD11b, CD18, CD44, CD34, CD25 and CD45. Distinct populations attributable to mast cells and eosinophils were recognized based on light scatters and CD117 positivity. Common antigens (CD18, CD45, CD44) and CD117 were detected in all cases; positivity for IgE and CD11b was found in 28 (82%) and 23 (68%) cases respectively, while CD34 and CD25 were occasionally expressed. A single multicolor tube (IgE/CD117/CD11b/CD21 /CDS) allowed the identification of mast cells in lymph nodes, showing a high correlation with cytology in quantifying mast cells infiltration. In conclusion, flow cytometric analysis can be applied to characterize canine MCTs and can be used to detect the presence of mast cells in lymph nodes. The immunophenotype abnormalities observed may be useful to confirm the neoplastic nature of such mast cells but the diagnostic usefulness of atypical antigen expression remains to be clarified. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：15 / 23

页数：9

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