Molecular cloning and functional expression of bottle-nosed dolphin (Tursiops truncatus) interleukin-1 receptor antagonist

被引：16
|
作者：
Inoue, Y
Itou, T
Jimbo, T
Syouji, Y
Ueda, K
Sakai, T
机构：
[1] Nihon Univ, Sch Vet Med, Dept Prevent Vet Med & Anim Hlth, Fujisawa, Kanagawa 2528510, Japan
[2] Nihon Univ, Coll Bioresource Sci, Dept Appl Biol Sci, Fujisawa, Kanagawa 2528510, Japan
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2001年 / 78卷 / 02期
关键词：
bottle-nosed dolphin; IL-1ra; IL-1; beta; sequence; expression;
D O I：
10.1016/S0165-2427(00)00263-4
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The bottle-nosed dolphin (Tursiops truncatus) interleukin-1 receptor antagonist IL-1ra cDNA was cloned from mitogen-stimulated peripheral blood mononuclear cells (PBMC) RNA utilizing the reverse transcription-polymerase chain reaction (RT-PCR). The sequence of this cDNA showed that dolphin IL-1ra clones contained open reading frames encoding 177 amino acids. Comparison of the deduced amino acids showed that dolphin IL-1ra sequence shared 87.6, 77.9, 77.4, 77.4, 76.4, and 75.8% similarity with the bovine, rabbit, equine, human, mouse. and rat IL-1ra sequences, respectively. Recombinant glutathione S-transferase (GST) dolphin IL-1ra produced in Escherichia coli (E. coli) was purified. This protein suppressed the cytostatic activity of dolphin IL-1 beta on A375S2 cells, indicating that the dolphin IL-1ra cDNA obtained in the present study encodes biologically active dolphin IL-1ra. (C) 2001 Elsevier Science B.V. All rights reserved.
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页码：131 / 141
页数：11
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