Extraction of sulfated agar from Gracilaria lemaneiformis using hydrogen peroxide-assisted enzymatic method

被引：36

作者：

Chen, Huijing ^{[1
,2
,3
,4
]}

Xiao, Qiong ^{[1
,3
,4
]}

Weng, Huifen ^{[1
,3
,4
]}

Zhang, Yonghui ^{[1
,2
,3
]}

Yang, Qiuming ^{[1
,2
,3
,4
]}

Xiao, Anfeng ^{[1
,2
,3
,4
]}

机构：

[1] Jimei Univ, Coll Food & Biol Engn, Xiamen 361021, Peoples R China

[2] Natl R&D Ctr Red Alga Proc Technol, Xiamen 361021, Fujian, Peoples R China

[3] Fujian Prov Key Lab Food Microbiol & Enzyme Engn, Xiamen 361021, Fujian, Peoples R China

[4] Fujian Prov Engn Technol Res Ctr Marine Funct Foo, Xiamen 361021, Fujian, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2020年 / 232卷

关键词：

Sulfated agar; Enzymatic method; H2O2-assisted enzymatic method; Filtration; Agar characterization; PHYSICOCHEMICAL PROPERTIES; SPECIES GRACILARIACEAE; ANTIOXIDANT ACTIVITY; GELLING PROPERTIES; ALKALI TREATMENT; GEL PROPERTIES; RED SEAWEEDS; RHODOPHYTA; POLYSACCHARIDES; QUALITY;

D O I：

10.1016/j.carbpol.2019.115790

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, an eco-friendly extraction method was explored to obtain high sulfate content agar and repair the deficiency of enzymatic extraction by taking full advantage of H2O2. The sulfate content of EHA (H2O2-assisted enzymatic extracted agar) reached 3.56 %, which is significantly higher than that of traditional alkali-extracted agar (AA, 1.8 %). Moreover, EHA exhibited lower viscosity (9.4 cP), which improved 26.6 % and 14 % of filtration and gel dehydration rates than EA (enzymatic extracted agar), respectively. Additionally, the physicochemical properties of the agars were evaluated and compared. Among these agars, EHA showed some favorable properties, such as high yield (16.08 %) and low dissolution temperature (88.9 degrees C). The surface of algae became smoother after treatment with H2O2 due to effective degradation of cellulose. Besides, mass spectrometry analysis revealed that EHA preserved a great amount of sulfate, while thermogravimetric analysis suggested that the thermal stability of EA and EHA both decreased.

引用

页数：11

共 56 条

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