Effect of NRG1, GDNF, EGF and NGF in the Migration of a Schwann Cell Precursor Line

被引：48

作者：

Cornejo, Martha ^{[1
]}

Nambi, Deborah ^{[1
]}

Walheim, Christopher ^{[1
]}

Somerville, Matthew ^{[1
]}

Walker, Jacquae ^{[1
]}

Kim, Lino ^{[1
]}

Ollison, Lauren ^{[1
]}

Diamante, Graciel ^{[1
]}

Vyawahare, Saurabh ^{[2
]}

de Bellard, Maria Elena ^{[1
]}

机构：

[1] Calif State Univ Northridge, Dept Biol, Northridge, CA 91330 USA

[2] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

NEUROCHEMICAL RESEARCH | 2010年 / 35卷 / 10期

关键词：

Schwann cell precursors; NeuregulinB1; NGF; GDNF; Cell migration; Chemoattraction; PERIPHERAL NERVOUS-SYSTEM; MAMMALIAN NEURAL CREST; GROWTH CONE GUIDANCE; MOLECULAR-BIOLOGY; ERBB3; RECEPTORS; AXON GUIDANCE; STEM-CELLS; IN-VIVO; ACTIVATION; EXPRESSION;

D O I：

10.1007/s11064-010-0225-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Schwann cells are the myelinating glia of the peripheral nervous system that originated during development from the highly motile neural crest. However, we do not know what the guidance signals for the Schwann cell precursors are. Therefore, we set to test some of the known neurotrophins that are expressed early in developing embryos and have been shown to be critical for the survival and patterning of developing glia and neurons. The goal of this study was to determine more specifically if GDNF, NRG1 and NGF are chemoattractants and/or chemokinetic molecules for a Schwann cell precursor line, the Spl201. We performed live chemoattraction assays, with imaging and also presented these molecules as part of their growing substrate. Our results show for the first time that GDNF and NRG1 are potent chemoattractive and chemokinetic molecules for these cells while NGF is a chemokinetic molecule stimulating their motility.

引用

页码：1643 / 1651

页数：9

共 48 条

[1] Chemotaxis in shallow gradients is mediated independently of PtdIns 3-kinase by biased choices between random protrusions [J].

Andrew, Natalie ;

Insall, Robert H. .

NATURE CELL BIOLOGY, 2007, 9 (02) :193-U91

[2] NERVE GROWTH-FACTOR AND ITS LOW-AFFINITY RECEPTOR PROMOTE SCHWANN-CELL MIGRATION [J].

ANTON, ES ;

WESKAMP, G ;

REICHARDT, LF ;

MATTHEW, WD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2795-2799

[3] An essential regulatory role for macrophage migration inhibitory factor in T-cell activation [J].

Bacher, M ;

Metz, CN ;

Calandra, T ;

Mayer, K ;

Chesney, J ;

Lohoff, M ;

Gemsa, D ;

Donnelly, T ;

Bucala, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :7849-7854

[4]

BARDE Y-A, 1990, Progress in Growth Factor Research, V2, P237, DOI 10.1016/0955-2235(90)90021-B

[5] PO IS AN EARLY MARKER OF THE SCHWANN-CELL LINEAGE IN CHICKENS [J].

BHATTACHARYYA, A ;

FRANK, E ;

RATNER, N ;

BRACKENBURY, R .

NEURON, 1991, 7 (05) :831-844

[6]

BHATTACHARYYA A, 1994, DEVELOPMENT, V120, P1411

[7] Cell migration and axon growth cone guidance in Caenorhabditis elegans [J].

Branda, CS ;

Stern, MJ .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (04) :479-484

[8] The ErbB2 and ErbB3 receptors and their ligand, neuregulin-1, are essential for development of the sympathetic nervous system [J].

Britsch, S ;

Li, L ;

Kirchhoff, S ;

Theuring, F ;

Brinkmann, V ;

Birchmeier, C ;

Riethmacher, D .

GENES & DEVELOPMENT, 1998, 12 (12) :1825-1836

[9]

BROCKES JP, 1979, IN VITRO CELL DEV B, V15, P772

[10] CELL LINEAGE ANALYSIS REVEALS MULTIPOTENCY OF SOME AVIAN NEURAL CREST CELLS [J].

BRONNERFRASER, M ;

FRASER, SE .

NATURE, 1988, 335 (6186) :161-164

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