Production, Optimization and Partial Characterization of Bacterial Cellulose from Gluconacetobacter xylinus TJU-D2

PurposeThe aim of this work was to optimize and characteristic a bacterial cellulose (BC) produced by Gluconoacetobacter xylinus TJU-D2.MethodsAn one-factor test was conducted, then response surface method was applied to optimize BC yield and discover the best production condition. Moreover, mechanical strength, water holding capacity, water absorption rate and water release rate of BC was determined.ResultsBased on Plackett-Burman experiment, glucose, ethanol and initial pH were found to be the most significant factors for BC production. Afterwards, effects of the three main factors on BC yield were further investigated by Box-Behnken design and the optimum composition was glucose 19.575 g/L, ethanol 3.85%, and initial pH 4.32. Optimum result showed that BC yield was increased to 4.820.23 g/L in 8th-day fermentation, 1.46-fold higher than before. Tensile strength reveals a Young's modulus of 18.640.23 MPa and tensile strength of up to 22.34 +/- 0.26 MPa.Conclusion p id=Par The strain produced a high level of BC with characteristics suitable for application in materials and medical areas.