Isolation and characterization of polymorphic microsatellite markers for the ariake icefish (Salanx ariakensis kishinouye)

被引:1
作者
Hu, Juan [1 ]
Ren, Sheng-Jie [1 ]
Fu, Cui-Zhang [2 ]
Gong, Xiao-Ling [1 ]
机构
[1] Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
[2] Fudan Univ, Minist Educ, Inst Biodivers Sci, Key Lab Biodivers Sci & Ecol Engn, Shanghai 200433, Peoples R China
基金
上海市自然科学基金;
关键词
Salanx ariakensis kishinouye; Microsatellite markers; Genetic diversity;
D O I
10.1007/s12686-010-9352-9
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Twenty microsatellite markers were isolated and characterized in the ariake icefish (Salanx ariakensis kishinouye). Analysis of 28 individuals from a single population revealed an average of 17.75 alleles per locus, which ranged from 8 to 26. The mean observed and expected heterozygosity (H(O) and H(E)) were 0.8410 and 0.9262, respectively. The average polymorphism information content was 0.8973, ranging from 0.7824 to 0.9521. These newly isolated markers will be useful in analyzing genetic diversity and genetic relations between the ariake icefish and its relatives.
引用
收藏
页码:315 / 318
页数:4
相关论文
共 9 条
[1]   A SIMPLE METHOD FOR RAPID ISOLATION OF MICROSATELLITES FROM YEAST ARTIFICIAL CHROMOSOMES [J].
BROWN, J ;
HARDWICK, LJ ;
WRIGHT, AF .
MOLECULAR AND CELLULAR PROBES, 1995, 9 (01) :53-57
[2]   Polymorphic microsatellite loci isolated from the topmouth culter (Culter alburnus Basilewsky) [J].
Chen, Shu-Qiang ;
Liu, Qi-Gen ;
Ren, Sheng-Jie ;
Gong, Xiao-Ling .
CONSERVATION GENETICS RESOURCES, 2009, 1 (01) :337-339
[3]   Phylogenetic relationships of salangid fishes (Osmeridae, Salanginae) with comments on phylogenetic placement of the salangids based on mitochondrial DNA sequences [J].
Fu, CZ ;
Luo, J ;
Wu, JH ;
López, JA ;
Zhong, Y ;
Lei, GC ;
Chen, JK .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2005, 35 (01) :76-84
[4]  
Raymond M, 1995, J HERED, V86, P8
[5]   ANALYZING TABLES OF STATISTICAL TESTS [J].
RICE, WR .
EVOLUTION, 1989, 43 (01) :223-225
[6]  
Sambrook J, 2002, MOL CLONING LAB MANU
[7]  
TANG ZP, 2000, DALIAN FISHERIES U, V15, P113
[8]  
Xie Y. H., 1997, CHIN J FISH, V10, P11
[9]  
YEH F., 1999, MICROSOFT WINDOWS BA