Expression of the Na+-K+-2Cl- cotransporter in the rat endolymphatic sac

被引：23
|
作者：
Akiyama, Kosuke [1 ]
Miyashita, Takenori [1 ]
Mori, Terushige [1 ]
Mori, Nozomu [1 ]
机构：
[1] Kagawa Univ, Fac Med, Dept Otolaryngol, Kagawa 7610793, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 364卷 / 04期
关键词：
endolymphatic sac; Na+-K+-2Cl(-) cotransporter; endolymphatic hydrops; ion transport; inner ear; Meniere's disease; endolymph; rat; In situ hybridization; polymerase chain reaction;
D O I：
10.1016/j.bbrc.2007.10.107
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The endolymphatic sac (ES) is a part of the membranous labyrinth that contains the cochlea, vestibular organs, and semicircular canals, and is believed to absorb endolymphatic fluid. Na+-K+-2Cl(-) (NKCC) is a cotransporter that occurs as two isoforms (NKCC-1 and NKCC-2). Especially, NKCC-2 is suggested to participate in ES endolymph absorption. In the present study, the expression and cellular localization of NKCC-1 and NKCC-2 in the rat ES were examined by RT-PCR and in situ hybridization, respectively. The findings indicate that both NKCC-1 and NKCC-2 are expressed in the rat ES and suggest that NKCC is involved in ES homeostasis. NKCC-2 may be particularly involved in endolymph absorption. This is the first report confirming NKCC expression in the ES. (C) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：913 / 917
页数：5
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