Humoral and cellular immune responses in mice against secreted and somatic antigens from a Corynebacterium pseudotuberculosis attenuated strain: Immune response against a C-pseudotuberculosis strain

被引：18

作者：

Costa Vale, Vera Lucia ^{[1
,5
]}

Silva, Marcos da Costa ^{[4
,5
]}

de Souza, Andreia Pacheco ^{[5
]}

Trindade, Soraya Castro ^{[3
,5
]}

de Moura-Costa, Lilia Ferreira ^{[2
]}

Nobre dos Santos-Lima, Ellen Karla ^{[5
]}

de Oliveira Nascimento, Ivana Lucia ^{[2
,5
]}

Pereira Cardoso, Hugo Saba ^{[1
]}

Marques, Edson de Jesus ^{[1
]}

Adrien Paule, Bruno Jean ^{[5
]}

Meyer Nascimento, Roberto Jose ^{[2
,5
]}

机构：

[1] Univ Estado Bahia, Dept Exact & Earth Sci, Campus 2, BR-48110100 Alagoinhas, BA, Brazil

[2] Univ Fed Bahia, Dept Biointeract, Ave Reitor Miguel Calmon S-N, BR-40110100 Salvador, BA, Brazil

[3] Feira de Santana State Univ, Dept Hlth, Ave Transnordestina S-N, BR-44036900 Feira De Santana, BA, Brazil

[4] Univ Estado Bahia, Dept Life Sci, Rua Silveira Martins 2555, BR-41150000 Salvador, BA, Brazil

[5] Univ Fed Bahia, Hlth Sci Inst, Immunol & Mol Biol Lab, Ave Reitor Miguel Calmon S-N, BR-40110100 Salvador, BA, Brazil

来源：

BMC VETERINARY RESEARCH | 2016年 / 12卷

关键词：

Corynebacterium pseudotuberculosis; Cytokines; BALB/c; IgG isotypes; INTERFERON-GAMMA PRODUCTION; NECROSIS-FACTOR-ALPHA; CASEOUS-LYMPHADENITIS; IFN-GAMMA; INFECTION; CYTOKINE; SHEEP; MACROPHAGES; RESISTANCE; GOATS;

D O I：

10.1186/s12917-016-0811-8

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic disease that affects goats and sheep. CL is characterized by the formation of granulomas in lymph nodes and other organs, such as the lungs and liver. Current knowledge of CL pathogenesis indicates that the induction of humoral and cellular immune responses are fundamental to disease control. The aim of this study was to evaluate the humoral and cellular immune responses in BALB/c mice inoculated with a C. pseudotuberculosis strain isolated in the state of Bahia, Brazil. Results: The lymphocyte proliferation and in vitro production of IFN-gamma, IL-4, IL-10, IL-12 and nitric oxide by spleen cells stimulated with secreted and somatic antigens from the studied strain were evaluated. IgG subclasses were also analyzed. Results showed a significant increase of Th1-profile cytokines after 60 days post-inoculation, as well as an important humoral response, represented by high levels of IgG2a and IgG1 against C. pseudotuberculosis. Conclusion: The T1 strain of C. pseudotuberculosis was shown to induce humoral and cellular immune responses in BALB/c mice, but, even at a dosage of 1x10(7) CFU, no signs of the disease were observed.

引用

页数：8

共 21 条

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Farjadian, Shirin
Hatam, Gholam Reza
Kalani, Mehdi
Sarkari, Bahador
IRANIAN JOURNAL OF IMMUNOLOGY, 2018, 15 (02) : 74 - 83

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