Simultaneous saccharification and fermentation of dilute alkaline-pretreated corn stover for enhanced butanol production by Clostridium saccharobutylicum DSM 13864

被引：30

作者：

Dong, Jin-Jun ^{[1
]}

Ding, Ji-Cai ^{[1
]}

Zhang, Yun ^{[1
]}

Ma, Li ^{[1
]}

Xu, Guo-Chao ^{[1
]}

Han, Rui-Zhi ^{[1
]}

Ni, Ye ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Biotechnol, Minist Educ, Key Lab Ind Biotechnol, 1800 Lihu Rd, Wuxi 214122, Jiangsu, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2016年 / 363卷 / 04期

基金：

中国国家自然科学基金; 中央高校基本科研业务费专项资金资助;

关键词：

simultaneous saccharification and fermentation; butanol; Clostridium saccharobutylicum; corn stover; ACID; HYDROLYSATE; BIOBUTANOL; BIOETHANOL; LIQUID; BATCH;

D O I：

10.1093/femsle/fnw003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Simultaneous saccharification and fermentation (SSF) process was applied for biobutanol production by Clostridium saccharobutylicum DSM 13864 from corn stover (CS). The key influential factors in SSF process, including corn steep liquor concentration, dry biomass and enzyme loading, SSF temperature, inoculation size and pre-hydrolysis time were optimized. In 5-L bioreactor with SSF process, butanol titer and productivity of 12.3 g/L and 0.257 g/L/h were achieved at 48 h, which were 20.6% and 21.2% higher than those in separate hydrolysis and fermentation (SHF), respectively. The butanol yield reached 0.175 g/g pretreated CS in SSF, representing 50.9% increase than that in SHF (0.116 g/g pretreated CS). This study proves the feasibility of efficient and economic production of biobutanol from CS by SSF.

引用

页数：6

共 26 条

[1] Production of biofuel using biomass as a sustainable biological resource [J].