A new O-methyltransferase for monolignol synthesis in Carthamus tinctorius

被引：2
作者：
Nakatsubo, Tomoyuki [1 ]
Ragamustari, Safendrri Komara [1 ]
Hattori, Takefumi [1 ,2 ]
Ono, Eiichiro [3 ]
Yamamura, Masaomi [1 ]
Li, Laigeng [4 ,5 ]
Chiang, Vincent L. [4 ]
Umezawa, Toshiaki [1 ,6 ]
机构：
[1] Kyoto Univ, Res Inst Sustainable Humanosphere, Uji, Kyoto 6110011, Japan
[2] Univ Tokushima, Inst Socioarts & Sci, Tokushima 7708502, Japan
[3] Suntory Global Innovat Ctr Ltd, Res Inst, Mishima, Osaka 6188503, Japan
[4] N Carolina State Univ, Coll Nat Resources, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA
[5] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China
[6] Kyoto Univ, Inst Sustainabil Sci, Uji, Kyoto 6110011, Japan
来源：
Plant Biotechnology | 2014年 / 31卷 / 05期
基金：
日本学术振兴会;
关键词：
O-Methyltransferase; Carthamus tinctorius; lignin; monolignol; flavonoid; AGROBACTERIUM-MEDIATED TRANSFORMATION; CAFFEIC ACID 3-O-METHYLTRANSFERASE; LIGNIN BIOSYNTHESIS; CINNAMATE/MONOLIGNOL PATHWAY; ORYZA-SATIVA; METHYLATION; SAFFLOWER; GENE; MATAIRESINOL; ANGIOSPERMS;
D O I：
10.5511/plantbiotechnology.14.0903a
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel type of O-methyltransferase (OMT) cDNA was isolated from maturing seeds of Carthamus tinctorius (safflower). The deduced sequence of the OMT protein showed moderate sequence identity (52%) with C. tinctorius 5-hydroxyconiferaldehyde O-methyltransferase 1 (CAldOMT1). Phylogenetic analysis showed that the novel OMT did not belong to the typical CAldOMT [=caffeic acid OMT (CAOMT)] cluster. The recombinant protein of the OMT catalyzed 3(or 5-) O-methylation of hydroxycinnamaldehydes and hydroxycinnamyl alcohols, while it showed only weak or moderate activity toward hydroxycinnamates and hydroxycinnamoyl coenzyme A esters. There fore, this OMT was designated as C. tinctorius 5-hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol OMT (CtAAOMT). The time profile of CtAAOMT gene expression in C. tinctorius matched the patterns of lignin accumulation. Taken together, our data strongly suggest that along with CtCAldOMT1, CtAAOMT is involved in biosynthesis of syringyl lignin.
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收藏
页码：545 / 553
页数：9
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