Assessment of genetic variability amongst cultivated populations of Khasi mandarin (Citrus reticulata Blanco) detected by ISSR

被引:6
作者
Kashyap, Karishma [1 ]
Bhagwat, Rasika M. [2 ]
Banu, Sofia [1 ]
机构
[1] Gauhati Univ, Dept Bioengn & Technol, GUIST, Gauhati 781014, India
[2] Western Reg Ctr, Teri, Mumbai 400614, Maharashtra, India
来源
PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION | 2021年 / 19卷 / 02期
关键词
AMOVA; gene flow; genetic diversity; Khasi mandarin; polymorphism; GERMPLASM COLLECTION; MOLECULAR MARKERS; DIVERSITY; L; DISTANCE; REGIONS; INDIA; SSR; DNA;
D O I
10.1017/S1479262121000162
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Khasi mandarin (Citrus reticulata Blanco) is a commercial mandarin variety grown in northeast India and one of the 175 Indian food items included in the global first food atlas. The cultivated plantations of Khasi mandarin grown prominently in the lower Brahmaputra valley of Assam, northeast India, have been genetically eroded. The lack in the efforts for conservation of genetic variability in this mandarin variety prompted diversity analysis of Khasi mandarin germplasm across the region. Thus, the study aimed to investigate genetic diversity and partitioning of the genetic variations within and among 92 populations of Khasi mandarin collected from 10 cultivated sites in Kamrup and Kamrup (M) districts of Assam, India, using Inter-Simple Sequence Repeat (ISSR) markers. The amplification of genomic DNA with 17 ISSR primers yielded 216 scorable DNA amplicons of which 177 (81.94%) were polymorphic. The average polymorphism information content was 0.39 per primer. The total genetic diversity (H-T = 0.28 +/- 0.03) was close to the diversity within the population (H-S = 0.20 +/- 0.01). A high mean coefficient of gene differentiation (G(ST) = 0.29) reflected a high level of gene flow (N-m = 1.22), indicating high genetic differentiation among the populations. Analysis of Molecular Variance (AMOVA) showed 78% of intra-population differentiation, 21% among the population and 1% among the districts. The obtained results indicate the existence of a high level of genetic diversity in the cultivated Khasi mandarin populations, indicating the need for preservation of each existing population to revive the dying out orchards in northeast India.
引用
收藏
页码:122 / 132
页数:11
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