Direct characterization of enzyme-substrate complexes by using electrosonic spray ionization mass spectrometry

被引：36
作者：
Wiseman, JM
Takáts, Z
Gologan, B
Davisson, VJ
Cooks, RG [1 ]
机构：
[1] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA
[2] Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 06期
关键词：
electrospray ionization; mass spectrometry; noncovalent interactions; proteins;
D O I：
10.1002/anie.200461672
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
It's a phase it's going through: Electrosonic spray ionization shows better desolvation efficiency than electrospray ionization and usually gives single-charge states when used to ionize noncovalent complexes of large biomolecules. The binary and ternary complexes of imidazole-3-glycerol phosphate synthase (IGPS) with its inhibitors appear to retain their native solution-phase conformations upon transfer into the gas phase (see picture).
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收藏
页码：913 / 916
页数：4
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