Influence of birefringence on polarization resolved nonlinear microscopy and collagen SHG structural imaging

被引：75

作者：

Ait-Belkacem, D. ^{[1
]}

Gasecka, A.

Munhoz, F.

Brustlein, S.

Brasselet, S.

机构：

[1] Aix Marseille Univ, Ecole Cent Marseille, Inst Fresnel, CNRS, F-13013 Marseille, France

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 14期

关键词：

2ND-HARMONIC GENERATION MICROSCOPY; RAT-TAIL TENDON; FLUORESCENCE POLARIZATION; ORIENTATION; POLARIMETRY; TISSUE; MONOLAYERS; SCATTERING; CRYSTALS; POLARITY;

D O I：

10.1364/OE.18.014859

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We analyze the influence of the anisotropy of molecular and biological samples on polarization resolved nonlinear microscopy imaging. We show in particular the detrimental influence of birefringence on Second Harmonic Generation (SHG) and Two-Photon Excited Fluorescence (TPEF) polarization resolved microscopy imaging, which, if not accounted for, can lead to an erroneous determination of the sample properties and thus to a misinterpretation of the read-out information. We propose a method to measure this birefringence and account for this effect in nonlinear polarization resolved experiments. (C) 2010 Optical Society of America

引用

页码：14859 / 14870

页数：12

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