Partial characterization of cotton plants expressing two toxin proteins from Bacillus thuringiensis:: relative toxin contribution, toxin interaction, and resistance management

被引：80

作者：

Greenplate, JT ^{[1
]}

Mullins, JW ^{[1
]}

Penn, SR ^{[1
]}

Dahm, A ^{[1
]}

Reich, BJ ^{[1
]}

Osborn, JA ^{[1
]}

Rahn, PR ^{[1
]}

Ruschke, L ^{[1
]}

Shappley, ZW ^{[1
]}

机构：

[1] Monsanto Co, St Louis, MO 63198 USA

来源：

JOURNAL OF APPLIED ENTOMOLOGY | 2003年 / 127卷 / 06期

关键词：

insect resistance management; lepidopteran efficacy; transgenic cotton; two Bt toxins;

D O I：

10.1046/j.1439-0418.2003.00766.x

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Laboratory studies were performed to characterize the lepidopteran toxicity of cotton plants expressing two different toxin proteins from Bacillus thuringiensis (Bt), in order to assess insect resistance management implications of a commercial, two-toxin transgenic cotton. An independent and additive interactive effect of two Bt delta-endotoxins expressed by the transgenic cotton variety 15985 was demonstrated by examining the responses of Heliothis virescens (F.), Helicoverpa zea (Boddie), and Spodoptera frugiperda (J.E. Smith) larvae to field- or greenhouse-grown tissue from genetic near-isolines, which expressed Cry1A only, Cry2Ab only, or both toxins. In all cases, the Cry2Ab component was the larger contributor to total toxicity in the two-toxin isoline. Toxin-specific, quantitative enzyme-linked immunosorbent assay (ELISA) tests confirmed that the levels of each toxin in tissues of the two-toxin isoline were not statistically different (P > 0.05) from the levels found in the corresponding tissues of the respective single-toxin isoline. Resistance management considerations were discussed. Considering the additive interaction of toxins, a relatively simple insect resistance-monitoring procedure was proposed for the monitoring of commercial cotton varieties expressing both toxins.

引用

页码：340 / 347

页数：8

共 60 条

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