Enhanced bacoside production in shoot cultures of Bacopa monnieri under the influence of abiotic elicitors

被引：51

作者：

Sharma, Munish ^{[1
]}

Ahuja, Ashok ^{[2
]}

Gupta, Rajinder ^{[3
]}

Mallubhotla, Sharada ^{[1
]}

机构：

[1] Shri Mata Vaishno Devi Univ, Sch Biotechnol, Katra 182320, India

[2] Indian Inst Integrat Med CSIR, Plant Tissue Culture Grp, Jammu 180001, India

[3] Indian Inst Integrat Med CSIR, Div Analyt Chem, Jammu 180001, India

来源：

NATURAL PRODUCT RESEARCH | 2015年 / 29卷 / 08期

关键词：

shoot biomass; elicitation; Brahmi; HPLC; secondary metabolite production; PLANT SECONDARY METABOLITES; METHYL JASMONATE; HYPERICUM-PERFORATUM; L; ELICITATION; ACID; GLYCOSIDES;

D O I：

10.1080/14786419.2014.986657

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO4) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in in vitro Bacopa monnieri shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74mgg(-1) dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91mgg(-1) DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45mgL(-1) of CuSO4 exhibited the highest bacoside content of 8.73mgg(-1) DW (similar to 1.42-fold higher) than in control cultures (6.14mgg(-1) DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in in vitro shoot cultures of this medicinally important herb known for its memory-enhancing properties.

引用

页码：745 / 749

页数：5

共 33 条

[1]

[Anonymous], 2011, Bull. Fac. Pharm. Cairo Univ., DOI DOI 10.1016/J.BFOPCU.2011.07.002

[2] Jasmonate signalling network in Arabidopsis thaliana:: crucial regulatory nodes and new physiological scenarios [J].

Balbi, Virginia ;

Devoto, Alessandra .

NEW PHYTOLOGIST, 2008, 177 (02) :301-318

[3] Production of plant secondary metabolites: a historical perspective [J].

Bourgaud, F ;

Gravot, A ;

Milesi, S ;

Gontier, E .

PLANT SCIENCE, 2001, 161 (05) :839-851

[4] Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum [J].

Coste, Ana ;

Vlase, Laurian ;

Halmagyi, Adela ;

Deliu, Constantin ;

Coldea, Gheorghe .

PLANT CELL TISSUE AND ORGAN CULTURE, 2011, 106 (02) :279-288

[5] In vitro production of radiolabeled red clover (Trifolium pratense) isoflavones [J].

Engelmann, Nancy J. ;

Reppert, Adam ;

Yousef, Gad ;

Rogers, Randy B. ;

Lila, Mary Ann .

PLANT CELL TISSUE AND ORGAN CULTURE, 2009, 98 (02) :147-156

[6] Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones [J].

Gadzovska, Sonja ;

Maury, Stephane ;

Delaunay, Alain ;

Spasenoski, Mirko ;

Joseph, Claude ;

Hagege, Daniel .

PLANT CELL TISSUE AND ORGAN CULTURE, 2007, 89 (01) :1-13

[7]

Gohil K. J., 2010, International Journal of Green Pharmacy, V4, P1

[8]

Grzegorczyk I., 2009, Herba Polonica, V55, P238

[9]

Harsahay M, 2012, INDIAN J PHARM, V44, P114

[10]

Kamal Kishore Kamal Kishore, 2012, Journal of Pharmacy Research, V5, P3139

← 1 2 3 4 →