Impact of paper filtered coffee on oxidative DNA-damage: Results of a clinical trial

被引：54

作者：

Misik, Miroslav ^{[1
]}

Hoelzl, Christine ^{[1
]}

Wagner, Karl-Heinz ^{[2
]}

Cavin, Christophe ^{[3
]}

Moser, Beate ^{[1
]}

Kundi, Michael ^{[4
]}

Simic, Tanja ^{[5
]}

Elbling, Leonilla ^{[1
]}

Kager, Nina ^{[1
]}

Ferk, Franziska ^{[1
]}

Ehrlich, Veronika ^{[1
]}

Nersesyan, Armen ^{[1
]}

Dusinska, Maria ^{[6
]}

Schilter, Benoit ^{[3
]}

Knasmueller, Siegfried ^{[1
]}

机构：

[1] Med Univ Vienna, Dept Inner Med 1, Inst Canc Res, A-1090 Vienna, Austria

[2] Univ Vienna, Dept Nutr Sci, A-1010 Vienna, Austria

[3] Nestle Res Ctr, Qual & Safety Dept, CH-1000 Lausanne, Switzerland

[4] Med Univ Vienna, Inst Hyg & Med Microbiol, A-1090 Vienna, Austria

[5] Univ Belgrade, Fac Med, Inst Biochem, Belgrade, Serbia

[6] Norwegian Inst Air Res, Ctr Ecol Econ, Kjeller, Norway

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2010年 / 692卷 / 1-2期

关键词：

Coffee; Antioxidant properties; SCGE; Human intervention trial; DNA protection; CHLOROGENIC ACID; COMET ASSAY; IN-VITRO; ANTIOXIDANT CAPACITY; DITERPENES CAFESTOL; LIPID-PEROXIDATION; TYROSINE NITRATION; COMPONENTS KAHWEOL; PHENOLIC-ACIDS; CONSUMPTION;

D O I：

10.1016/j.mrfmmm.2010.08.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Coffee is among the most frequently consumed beverages worldwide and epidemiological studies indicate that its consumption is inversely related to the incidence of diseases in which reactive oxygen species (ROS) are involved (liver cirrhosis, certain forms of cancer and neurodegenerative disorders). It has been postulated that antioxidant properties of coffee may account for this phenomenon. To find out if consumption of paper filtered coffee which is the most widely consumed form in Central Europe and the US protects humans against oxidative DNA-damage, a controlled intervention trial with a cross-over design was conducted in which the participants (n = 38) consumed 800 ml coffee or water daily over 5 days. DNA-damage was measured in peripheral lymphocytes in single cell gel electrophoresis assays. The extent of DNA-migration attributable to formation of oxidised purines (formamidopyrimidine glycosylase sensitive sites) was decreased after coffee intake by 12.3% (p = 0.006). Biochemical parameters of the redox status (malondialdehyde, 3-nitrotyrosine and the total antioxidant levels in plasma, glutathione concentrations in blood, intracellular ROS levels and the activities of superoxide dismutase and glutathione peroxidase in lymphocytes) were not markedly altered at the end of the trial, also the urinary 8-isoprostaglandine F2 alpha concentrations were not affected. Overall, the results indicate that coffee consumption prevents endogenous formation of oxidative DNA-damage in human, this observation may be causally related to beneficial health effects of coffee seen in earlier studies. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：42 / 48

页数：7

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