Production of human CD59-transgenic pigs by embryonic germ cell nuclear transfer

被引：13

作者：

Ahn, Kwang Sung ^{[2
]}

Won, Ji Young ^{[2
]}

Park, Jin-Ki ^{[3
]}

Sorrell, Alice M. ^{[2
]}

Heo, Soon Young ^{[1
]}

Kang, Jee Hyun ^{[1
]}

Woo, Jae-Seok ^{[3
]}

Choi, Bong-Hwan ^{[4
]}

Chang, Won-Kyong ^{[3
]}

Shim, Hosup ^{[1
,5
]}

机构：

[1] Dankook Univ, Dept Nanobiomed Sci, Cheonan 330714, Chungnam, South Korea

[2] Dankook Univ, Sch Med, Dept Physiol, Cheonan 330714, Chungnam, South Korea

[3] Natl Inst Anim Sci, Anim Biotechnol Div, Suwon, South Korea

[4] Natl Inst Anim Sci, Genom & Bioinformat Div, Suwon, South Korea

[5] Dankook Univ, Inst Tissue Regenerat Engn, Cheonan 330714, Chungnam, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 400卷 / 04期

关键词：

Nuclear transfer; Embryonic germ cell; Human CD59; Transgenic pig; STEM-CELLS; CLONED PIGS; COMPLEMENT; XENOTRANSPLANTATION; CULTURE; FIBROBLASTS; INHIBITION; EXPRESSION; REJECTION; PROSPECTS;

D O I：

10.1016/j.bbrc.2010.08.125

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was performed to produce transgenic pigs expressing the human complement regulatory protein CD59 (hCD59) using the nuclear transfer (NT) of embryonic germ (EG) cells, which are undifferentiated stem cells derived from primordial germ cells. Because EG cells can be cultured indefinitely in an undifferentiated state, they may provide an inexhaustible source of nuclear donor cells for NT to produce transgenic pigs. A total of 1980 NT embryos derived from hCD59-transgenic EG cells were transferred to ten recipients, resulting in the birth of fifteen piglets from three pregnancies. Among these offspring, ten were alive without overt health problems. Based on PCR analysis, all fifteen piglets were confirmed as hCD59 transgenic. The expression of the hCD59 transgene in the ten living piglets was verified by RTPCR. Western analysis showed the expression of the hCD59 protein in four of the ten RT-PCR-positive piglets. These results demonstrate that hCD59-transgenic pigs could effectively be produced by EG cell NT and that such transgenic pigs may be used as organ donors in pig-to-human xenotransplantation. (c) 2010 Elsevier Inc. All rights reserved.

引用

页码：667 / 672

页数：6

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