A novel method to control carryover contamination in isothermal nucleic acid amplification

被引：39

作者：

Ma, Cuiping ^{[1
,2
]}

Wang, Fuxin ^{[1
]}

Wang, Xiudan ^{[1
]}

Han, Lingzhi ^{[2
]}

Jing, Hao ^{[1
]}

Zhang, Heng ^{[3
]}

Shi, Chao ^{[4
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China

[2] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Sensor Anal Tumor Marker, Minist Educ, Qingdao 266042, Peoples R China

[3] Shenzhen Entry Exit Inspect & Quarantine Bur, Tech Ctr Food Inspect & Quarantine, Shenzhen 518000, Peoples R China

[4] Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 77期

基金：

中国国家自然科学基金;

关键词：

REAL-TIME PCR; COLORIMETRIC DETECTION; DNA AMPLIFICATION; FALSE POSITIVES; LAMP; SEQUENCES; DIAGNOSIS; PRODUCTS; SAMPLES;

D O I：

10.1039/c7cc06469a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We developed a novel method to control carryover contamination in loop-mediated isothermal amplification (LAMP) by primer engineering to carry recognition sites for a restriction endonuclease, providing a robust ability to eliminate carryover contaminants.

引用

页码：10696 / 10699

页数：4

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