Rapid Detection of the Commonly Encountered Carbapenemases (New Delhi Metallo-β-Lactamase, OXA-48/181) Directly from Various Clinical Samples using Multiplex Real-Time Polymerase Chain Reaction Assay
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Kazi, Mubin
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PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, IndiaPD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
Kazi, Mubin
[1
]
Khot, Rukhsar
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PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, IndiaPD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
Khot, Rukhsar
[1
]
Shetty, Anjali
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PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, IndiaPD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
Shetty, Anjali
[1
]
Rodrigues, Camilla
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PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, IndiaPD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
Rodrigues, Camilla
[1
]
机构:
[1] PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
Background: Resistance due to New Delhi metallo-beta-lactamase (NDM) and OXA-48/181 continues to emerge as a threat which is associated with nosocomial outbreaks and is a serious healthcare concern. Phenotypic detection being laborious and time-consuming requires rapid detection of NDM and OXA-48/181, which is achieved through real-time polymerase chain reaction (RT-PCR). Materials and Methods: In this study, RT-PCR assay was developed to simultaneously detect NDM and OXA-48/181. The assay was validated on 102 non-duplicate, phenotypically characterised clinical samples. Results: The assay showed a sensitivity and specificity of 97% and 100% for the detection of carbapenemases in comparison to conventional PCR. The in-house developed multiplex RT-PCR would help to rule-in the presence of NDM and OXA-48/181. Conclusions: Rapid detection of these carbapenemases would be assist in better patient management, in terms of accurate antimicrobial treatment, help in cohorting infected from uninfected patient to prevent spread.
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Hindiyeh, Musa
;
Smollen, Gill
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Smollen, Gill
;
Grossman, Zehava
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Grossman, Zehava
;
Ram, Daniela
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Ram, Daniela
;
Davidson, Yehudit
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Davidson, Yehudit
;
Mileguir, Fernando
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Mileguir, Fernando
;
Vax, Marina
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Vax, Marina
;
Ben David, Debbie
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Ben David, Debbie
;
Tal, Ilana
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Tal, Ilana
;
Rahav, Galia
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Rahav, Galia
;
Shamiss, Ari
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机构:Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Shamiss, Ari
;
Mendelson, Ella
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Mendelson, Ella
;
Keller, Nathan
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Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Khan, Asad U.
;
Maryam, Lubna
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Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Maryam, Lubna
;
Zarrilli, Raffaele
论文数: 0引用数: 0
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Univ Napoli Federico II, Dept Publ Hlth, Naples, Italy
CEINGE Biotecnol Avanzate, Naples, ItalyAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
机构:
Emory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USAEmory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA
Lutgring, Joseph D.
;
Limbago, Brandi M.
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Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Clin & Environm Microbiol Branch, Atlanta, GA USAEmory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA
机构:
Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Hindiyeh, Musa
;
Smollen, Gill
论文数: 0引用数: 0
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机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Smollen, Gill
;
Grossman, Zehava
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Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Grossman, Zehava
;
Ram, Daniela
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机构:
Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Ram, Daniela
;
Davidson, Yehudit
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机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Davidson, Yehudit
;
Mileguir, Fernando
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机构:
Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Mileguir, Fernando
;
Vax, Marina
论文数: 0引用数: 0
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机构:
Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Vax, Marina
;
Ben David, Debbie
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Ben David, Debbie
;
Tal, Ilana
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h-index: 0
机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Tal, Ilana
;
Rahav, Galia
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Rahav, Galia
;
Shamiss, Ari
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h-index: 0
机构:Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Shamiss, Ari
;
Mendelson, Ella
论文数: 0引用数: 0
h-index: 0
机构:
Chaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
Mendelson, Ella
;
Keller, Nathan
论文数: 0引用数: 0
h-index: 0
机构:
Tel Aviv Univ, Sheba Med Ctr, Infect Dis Unit, Microbiol Lab, Tel Hashomer, IsraelChaim Sheba Med Ctr, Minist Hlth, Publ Hlth Serv, Cent Virol Lab, IL-52621 Tel Hashomer, Israel
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Khan, Asad U.
;
Maryam, Lubna
论文数: 0引用数: 0
h-index: 0
机构:
Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, IndiaAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
Maryam, Lubna
;
Zarrilli, Raffaele
论文数: 0引用数: 0
h-index: 0
机构:
Univ Napoli Federico II, Dept Publ Hlth, Naples, Italy
CEINGE Biotecnol Avanzate, Naples, ItalyAligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Med Microbiol & Mol Biol Lab, Aligarh 202002, Uttar Pradesh, India
机构:
Emory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USAEmory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA
Lutgring, Joseph D.
;
Limbago, Brandi M.
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Dis Control & Prevent, Div Healthcare Qual Promot, Clin & Environm Microbiol Branch, Atlanta, GA USAEmory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA