Rapid Detection of the Commonly Encountered Carbapenemases (New Delhi Metallo-β-Lactamase, OXA-48/181) Directly from Various Clinical Samples using Multiplex Real-Time Polymerase Chain Reaction Assay

被引:0
作者
Kazi, Mubin [1 ]
Khot, Rukhsar [1 ]
Shetty, Anjali [1 ]
Rodrigues, Camilla [1 ]
机构
[1] PD Hinduja Hosp & Med Res Ctr, Dept Microbiol, Veer Savarkar Marg West, Bombay 400016, Maharashtra, India
关键词
Carbapenemases; infection control; New Delhi metallo-beta-lactunase; OXA-48-181; patient management; real-time polymerase chain reaction; rule-in; RESISTANT-ENTEROBACTERIACEAE; MOLECULAR CHARACTERIZATION; KLEBSIELLA-PNEUMONIAE; GLOBAL SURVEILLANCE; ESCHERICHIA-COLI; GENES; PCR; BLA(KPC); SPREAD;
D O I
10.4103/ijmm.IJMM_18_324
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background: Resistance due to New Delhi metallo-beta-lactamase (NDM) and OXA-48/181 continues to emerge as a threat which is associated with nosocomial outbreaks and is a serious healthcare concern. Phenotypic detection being laborious and time-consuming requires rapid detection of NDM and OXA-48/181, which is achieved through real-time polymerase chain reaction (RT-PCR). Materials and Methods: In this study, RT-PCR assay was developed to simultaneously detect NDM and OXA-48/181. The assay was validated on 102 non-duplicate, phenotypically characterised clinical samples. Results: The assay showed a sensitivity and specificity of 97% and 100% for the detection of carbapenemases in comparison to conventional PCR. The in-house developed multiplex RT-PCR would help to rule-in the presence of NDM and OXA-48/181. Conclusions: Rapid detection of these carbapenemases would be assist in better patient management, in terms of accurate antimicrobial treatment, help in cohorting infected from uninfected patient to prevent spread.
引用
收藏
页码:369 / 375
页数:7
相关论文
共 29 条
[11]  
González LJ, 2016, NAT CHEM BIOL, V12, P516, DOI [10.1038/nchembio.2083, 10.1038/NCHEMBIO.2083]
[12]   A framework for global surveillance of antibiotic resistance [J].
Grundmann, Hajo ;
Klugman, Keith P. ;
Walsh, Timothy ;
Ramon-Pardo, Pilar ;
Sigauque, Betuel ;
Khan, Wasif ;
Laxminarayan, Ramanan ;
Heddini, Andreas ;
Stelling, John .
DRUG RESISTANCE UPDATES, 2011, 14 (02) :79-87
[13]   Rapid detection of blaKPC carbapenemase genes by real-time PCR [J].
Hindiyeh, Musa ;
Smollen, Gill ;
Grossman, Zehava ;
Ram, Daniela ;
Davidson, Yehudit ;
Mileguir, Fernando ;
Vax, Marina ;
Ben David, Debbie ;
Tal, Ilana ;
Rahav, Galia ;
Shamiss, Ari ;
Mendelson, Ella ;
Keller, Nathan .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (09) :2879-2883
[14]   Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories [J].
Hrabak, J. ;
Chudackova, E. ;
Papagiannitsis, C. C. .
CLINICAL MICROBIOLOGY AND INFECTION, 2014, 20 (09) :839-853
[15]   Dual-tubed multiplex-PCR for molecular characterization of carbapenemases isolated among Acinetobacter spp. and Pseudomonas spp. [J].
Kazi, M. ;
Nikam, C. ;
Shetty, A. ;
Rodrigues, C. .
JOURNAL OF APPLIED MICROBIOLOGY, 2015, 118 (05) :1096-1102
[16]   Molecular characterization of carbapenem-resistant Enterobacteriaceae at a tertiary care laboratory in Mumbai [J].
Kazi, M. ;
Drego, L. ;
Nikam, C. ;
Ajbani, K. ;
Soman, R. ;
Shetty, A. ;
Rodrigues, C. .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2015, 34 (03) :467-472
[17]   An Xpert screen to identify carbapenemases [J].
Kazi, Mubin ;
Nikam, Chaitali ;
Shetty, Anjali ;
Rodrigues, Camilla .
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2016, 34 (02) :216-218
[18]   The Carbapenemase Menace: Do Dual Mechanisms Code for More Resistance? [J].
Kazi, Mubin ;
Shetty, Anjali ;
Rodrigues, Camilla .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2015, 36 (01) :116-117
[19]   Structure, Genetics and Worldwide Spread of New Delhi Metallo-β-lactamase (NDM): a threat to public health [J].
Khan, Asad U. ;
Maryam, Lubna ;
Zarrilli, Raffaele .
BMC MICROBIOLOGY, 2017, 17
[20]   The Problem of Carbapenemase-Producing-Carbapenem-Resistant-Enterobacteriaceae Detection [J].
Lutgring, Joseph D. ;
Limbago, Brandi M. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2016, 54 (03) :529-534